College of Resources and Environment, Northeast Agricultural University, Harbin 150030, Heilongjiang, PR China.
Heilongjiang University of Traditional Chinese Medicine, Harbin 150030, Heilongjiang, PR China.
Ecotoxicol Environ Saf. 2018 Nov 15;163:205-214. doi: 10.1016/j.ecoenv.2018.07.064. Epub 2018 Jul 25.
Despite many reports of the use of biodegradation to remove contaminants, the biodegradation of polycyclic aromatic hydrocarbons (PAHs) is challenging because of the hydrophobicities and low aqueous solubilities of most PAHs. In this study, phenanthrene (PHE) was used as a sole carbon and energy source to screen and identify Achromobacter sp. LH-1 for the production of biodemulsifiers that enhance the bioavailability and solubilization of PAHs. LH-1 achieved a 94% degradation rate and a 40% mineralization rate with 100 mg/L PHE. Additionally, LH-1 degraded various PAHs, and the factors that influenced the growth and PAHs degradation activity of LH-1 were not only the toxicities and structures of the substances but also the acclimation of LH-1 to these substances. Three kinetic models were used to describe the fermentation processes of cell growth, product formation and substrate degradation over time. Finally, multiple PHE degradation pathways were proposed to be utilized by strain LH-1.
尽管有许多关于利用生物降解去除污染物的报道，但由于大多数多环芳烃（PAHs）的疏水性和低水溶解度，PAHs 的生物降解具有挑战性。在这项研究中，菲（PHE）被用作唯一的碳源和能源，以筛选和鉴定出产生生物乳化剂的不动杆菌属 LH-1，以提高 PAHs 的生物利用度和增溶度。LH-1 以 100mg/L PHE 实现了 94%的降解率和 40%的矿化率。此外，LH-1 还降解了各种 PAHs，影响 LH-1 生长和 PAHs 降解活性的因素不仅是物质的毒性和结构，还有 LH-1 对这些物质的适应能力。三种动力学模型被用来描述细胞生长、产物形成和底物降解随时间的发酵过程。最后，提出了多种可能被 LH-1 菌株利用的 PHE 降解途径。
