Bajelani Sara, Enayatizamir Naeimeh, Agha Ali Beheshti Ale, Sharifi Rouhallah
Department of Soil Science and Engineering, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Department of Soil Science, Faculty of Agriculture, Razi University, Kermanshah, Iran.
J Environ Health Sci Eng. 2023 Aug 15;21(2):417-428. doi: 10.1007/s40201-023-00868-9. eCollection 2023 Dec.
Potential of B27Pet, B48Pet and to produce biosurfactant using four different carbon sources (naphthalene, hexadecane, diesel and petroleum crude oil) was investigated. Removal of petroleum crude oil from aqueous culture and degradation of diesel were also determined using single and mixed culture of strains. The biofilm existence in single and mixed culture of strains was considered using naphthalene, hexadecane and diesel in culture medium. Cell surface hydrophobicity of was higher than other isolates which also showed maximum surface tension reduction and emulsification index. As a whole, remarkable biosurfactant production occurred using petroleum crude oil as a carbon source in medium. was found to be more robust than other tested strains in removal efficiency of crude oil due to its biosurfactant production capability. Statistically significant positive correlation was observed between biofilm existence and surface tension using diesel and hexadecane as carbon source. Overall diesel biodegradation efficiency by the mix culture of three applied strains was about 75% within a short period of time (10 days) which was accompanied with high biofilm production.
研究了B27Pet、B48Pet利用四种不同碳源（萘、十六烷、柴油和石油原油）生产生物表面活性剂的潜力。还使用菌株的单一和混合培养测定了从水培物中去除石油原油以及柴油的降解情况。在培养基中使用萘、十六烷和柴油来考察菌株单一和混合培养中的生物膜存在情况。B27Pet的细胞表面疏水性高于其他分离株，其表面张力降低和乳化指数也最高。总体而言，以石油原油作为培养基中的碳源时，能产生显著的生物表面活性剂。由于其生物表面活性剂生产能力，B27Pet在原油去除效率方面比其他测试菌株更强。以柴油和十六烷作为碳源时，观察到生物膜存在与表面张力之间存在统计学上显著的正相关。在所应用的三种菌株的混合培养下，柴油在短时间内（十天）的总体生物降解效率约为75%，同时伴有大量生物膜产生。
