Key Laboratory of Industrial Biotechnology, Ministry of Education and School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China; Jiangsu Provisional Research Center for Bioactive Product Processing Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.
The Key Laboratory of Carbohydrate Chemistry & Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Bioresour Technol. 2018 Nov;267:578-583. doi: 10.1016/j.biortech.2018.07.104. Epub 2018 Jul 21.
Coniferyl alcohol is a valuable chemical. However, the current approaches to obtain coniferyl alcohol are either unefficient or expensive. Penicillium simplicissimum vanillyl alcohol oxidase (PsVAO) can be used to produce coniferyl alcohol. However, PsVAO intrinsically produces harmful byproduct HO. Utilizing catalase to decompose HO is a potential straightforward approach; however, catalase can also exhibit peroxidase activity to facilitate coniferyl alcohol over-oxidation. In this study, catalases exhibiting both high catalase activity and low peroxidase activity were found out, and introduced into the bioconversion systems. Our results showed that eliminating HOin situ released HO inhibition of PsVAO, improved coniferyl alcohol production and eliminated coniferyl alcohol over-oxidation. Finally, coniferyl alcohol titer, molar yield, and productivity reached 22.9 g/L, 78.7%, and 0.5 g/(L × h) respectively. An efficient coniferyl alcohol production method was developed by overcoming the intrinsic disadvantages of PsVAO.
松柏醇是一种有价值的化学物质。然而，目前获得松柏醇的方法要么效率低下，要么成本高昂。产黄青霉香草醇氧化酶（PsVAO）可用于生产松柏醇。然而，PsVAO 会内在地产生有害的副产物 HO。利用过氧化氢酶分解 HO 是一种潜在的直接方法；然而，过氧化氢酶也具有过氧化物酶活性，可促进松柏醇过度氧化。在这项研究中，发现了具有高过氧化氢酶活性和低过氧化物酶活性的过氧化氢酶，并将其引入生物转化系统。我们的结果表明，原位消除 HO 可释放 HO 对 PsVAO 的抑制作用，提高松柏醇的产量并消除松柏醇的过度氧化。最终，松柏醇的浓度、摩尔产率和生产效率分别达到 22.9g/L、78.7%和 0.5g/（L×h）。通过克服 PsVAO 的内在缺点，开发了一种有效的松柏醇生产方法。
