Saad Alaaeldin Mohamed, Askora Ahmed, Soliman Ahmed Mahrous, Nariya Hirofumi, Kawasaki Takeru, Fujie Makoto, Shimamoto Tadashi, Yamada Takashi
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, 739-8530, Japan.
Department of Zoonoses, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
Arch Virol. 2018 Nov;163(11):3207-3210. doi: 10.1007/s00705-018-3971-y. Epub 2018 Jul 28.
A novel lytic bacteriophage, Escherichia phage EcS1, was isolated from sewage samples collected in Higashi-Hiroshima, Japan. The complete genome sequence of EcS1 was determined using the Illumina Miseq System. The whole genome of EcS1 was found to be 175,437 bp in length with a mean G+C content of 37.8%. A total of 295 genes were identified as structural, functional, and hypothetical genes. BLAST analysis of the EcS1 genomic sequence revealed the highest identity (79%; query cover of 73-74%) to three T4-related phages that infect Serratia sp. ATCC 39006. Host range experiments revealed that EcS1 has lytic effects on three pathogenic strains of Shigella spp. and a pathogenic strain of Salmonella enterica as well as on E. coli strains. However, two strains of Serratia marcescens showed resistance to this phage. Phylogenetic trees for phage tail fiber protein sequences revealed that EcS1 is closely related to Enterobacteriaceae-infecting phages. Thus, EcS1 is a novel phage that infects several pathogenic strains of the family Enterobacteriaceae.
一种新型裂解性噬菌体——大肠杆菌噬菌体EcS1，是从日本东广岛采集的污水样本中分离出来的。使用Illumina Miseq系统测定了EcS1的完整基因组序列。发现EcS1的全基因组长度为175,437 bp，平均G+C含量为37.8%。总共鉴定出295个基因，包括结构基因、功能基因和假设基因。对EcS1基因组序列的BLAST分析显示，它与三种感染粘质沙雷氏菌ATCC 39006的T4相关噬菌体具有最高的同源性（79%；查询覆盖率为73-74%）。宿主范围实验表明，EcS1对三种志贺氏菌属致病菌株、一种肠炎沙门氏菌致病菌株以及大肠杆菌菌株具有裂解作用。然而，两株粘质沙雷氏菌对这种噬菌体表现出抗性。噬菌体尾纤维蛋白序列的系统发育树表明，EcS1与感染肠杆菌科的噬菌体密切相关。因此，EcS1是一种新型噬菌体，可感染肠杆菌科的几种致病菌株。
