Fromm M E, Taylor L P, Walbot V
Nature. 1986;319(6056):791-3. doi: 10.1038/319791a0.
The graminaceous monocots, including the economically important cereals, seem to be refractory to infection by Agrobacterium tumefaciens, a natural gene transfer system that has been successfully exploited for transferring foreign genes into higher plants. Therefore, direct transfer techniques that are potentially applicable to all plant species have been developed using a few dicot and monocot species as model systems. One of these techniques, electroporation, uses electrical pulses of high field strength to permeabilize cell membranes reversibly so as to facilitate the transfer of DNA into cells. Electroporation-mediated gene transfer has resulted in stably transformed animal cells and transient gene expression in monocot and dicot plant cells. Here we report that electroporation-mediated DNA transfer of a chimaeric gene encoding neomycin phosphotransferase results in stably transformed maize cells that are resistant to kanamycin.
包括具有重要经济价值的谷类作物在内的禾本科单子叶植物，似乎对根癌农杆菌的感染具有抗性，根癌农杆菌是一种已成功用于将外源基因导入高等植物的天然基因转移系统。因此，人们利用一些双子叶植物和单子叶植物作为模式系统，开发了可能适用于所有植物物种的直接转移技术。其中一种技术是电穿孔，它利用高场强电脉冲使细胞膜可逆地通透化，以便于将DNA导入细胞。电穿孔介导的基因转移已在动物细胞中实现稳定转化，并在单子叶植物和双子叶植物细胞中实现瞬时基因表达。在此，我们报告编码新霉素磷酸转移酶的嵌合基因通过电穿孔介导的DNA转移，可产生对卡那霉素具有抗性的稳定转化玉米细胞。
