1Laboratory of Veterinary Bacteriology, Department of Microbiology and Parasitology, Universidade Federal Fluminense, Niterói, RJ, Brazil.
2Laboratory of Epidemiology and Molecular Systematics, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro 21040-900, Brazil.
J Med Microbiol. 2018 Sep;67(9):1361-1367. doi: 10.1099/jmm.0.000806. Epub 2018 Jul 30.
Leptospira interrogans serogroup Icterohaemorrhagiae strains have been described as causing disease in both humans and animals and as being present worldwide. Icterohaemorrhagiae and Copenhageni serovars are known to cause severe disease in their hosts, and zoonotic outbreaks have been described. The genetic similarity among the strains of these serovars is known. However, it has not yet been demonstrated whether major clonal subpopulation in humans, strain Fiocruz L1-130-like, can circulate among other hosts.
We performed genetic characterization of Brazilian serogroup Icterohaemorrhagiae strains of dog and rat origin by secY sequencing, variable-number tandem-repeat, multilocus sequence type and multi-spacer typing analysis.
The strains were found to be identical among themselves and to strain Fiocruz L1-130. We suggest that the major strain of L. interrogans serogroup Icterohaemorrhagiae, Fiocruz L1-130, is widely distributed in Brazil in different hosts with substantial zoonotic potential.
Understanding the circulation of strain Fiocruz L1-130 is important for the implementation of appropriate control measures. Its circulation highlights the need to treat leptospirosis caused by L. interrogans serogroup Icterohaemorrhagiae as a zoonosis that acts in the human-animal-environment interface, as per the One Health approach.
已发现伊氏钩端螺旋体血清群 Icterohaemorrhagiae 菌株可引起人类和动物的疾病，并在全球范围内存在。已知 Icterohaemorrhagiae 和 Copenhageni 血清型可在其宿主中引起严重疾病，并且已经描述了人畜共患暴发。这些血清型菌株之间的遗传相似性是已知的。然而，尚未证明人类中主要的克隆亚群 Fiocruz L1-130 样菌株是否可以在其他宿主中传播。
我们通过 secY 测序、可变数串联重复、多位点序列分型和多位点间隔分型分析，对来自狗和鼠的巴西血清群 Icterohaemorrhagiae 菌株进行遗传特征分析。
发现这些菌株彼此之间以及与 Fiocruz L1-130 菌株完全相同。我们建议，广泛分布于巴西不同宿主中的主要伊氏钩端螺旋体血清群 Icterohaemorrhagiae 菌株 Fiocruz L1-130 具有很大的人畜共患病潜力。
了解 Fiocruz L1-130 菌株的传播情况对于实施适当的控制措施非常重要。其传播情况突出表明，需要按照“同一健康”方法，将由伊氏钩端螺旋体血清群 Icterohaemorrhagiae 引起的钩端螺旋体病视为在人类-动物-环境界面起作用的人畜共患病。
