USC1233 INRA/VAS, Equipe PERS, Etablissement VetAgro Sup, Campus de Lyon, Marcy l'Etoile, France.
J Clin Microbiol. 2014 Feb;52(2):564-71. doi: 10.1128/JCM.02482-13. Epub 2013 Dec 11.
Leptospirosis is a worldwide zoonosis which is responsible for the typical form of Weil's disease. The epidemiological surveillance of the Leptospira species agent is important for host prevalence control. Although the genotyping methods have progressed, the identification of some serovars remains ambiguous. We investigated the multispacer sequence typing (MST) method for genotyping strains belonging to the species Leptospira interrogans, which is the main agent of leptospirosis worldwide. A total of 33 DNA samples isolated from the reference strains of L. interrogans serogroups Icterohaemorrhagiae, Australis, Canicola, and Grippotyphosa, which are the most prevalent serogroups in France, were analyzed by both the variable-number tandem-repeat (VNTR) and MST methods. An MST database has been constructed from the DNA of these reference strains to define the MST profiles. The MST profiles corroborated with the VNTR results. Moreover, the MST analysis allowed the identification at the serovar level or potentially to the isolate level for strains belonging to L. interrogans serovar Icterohaemorrhagiae, which then results in a higher resolution than VNTR (Hunter-Gaston index of 0.94 versus 0.68). Regarding L. interrogans serogroups Australis, Canicola, and Grippotyphosa, the MST and VNTR methods similarly identified the genotype. The MST method enabled the acquisition of simple and robust results that were based on the nucleotide sequences. The MST identified clinical isolates in correlation with the reference serovar profiles, thus permitting an epidemiological surveillance of circulating L. interrogans strains, especially for the Icterohaemorrhagiae serogroup, which includes the most prevalent strains of public health interest.
钩端螺旋体病是一种全球性的动物传染病，是韦尔氏病的典型形式。钩端螺旋体物种的流行病学监测对于宿主流行的控制非常重要。尽管基因分型方法已经取得了进展，但一些血清型的鉴定仍然存在模糊性。我们研究了多间隔序列分型（MST）方法，用于对属于钩端螺旋体属的菌株进行基因分型，该属是全世界钩端螺旋体病的主要病原体。从法国最常见的血清组 Icterohaemorrhagiae、Australis、Canicola 和 Grippotyphosa 的参考菌株中分离出的 33 个 DNA 样本，通过可变数串联重复（VNTR）和 MST 方法进行了分析。从这些参考菌株的 DNA 构建了一个 MST 数据库，以定义 MST 图谱。MST 图谱与 VNTR 结果相符。此外，MST 分析允许对属于 Icterohaemorrhagiae 血清型的菌株进行血清型水平或潜在的分离株水平的鉴定，这比 VNTR 具有更高的分辨率（Hunter-Gaston 指数为 0.94 对 0.68）。关于 Australis、Canicola 和 Grippotyphosa 血清组的钩端螺旋体，MST 和 VNTR 方法同样可以识别基因型。MST 方法能够获得基于核苷酸序列的简单而可靠的结果。MST 与参考血清型图谱相关联，鉴定出了临床分离株，从而能够对循环钩端螺旋体菌株进行流行病学监测，特别是对包括最常见的公共卫生关注菌株的 Icterohaemorrhagiae 血清组进行监测。
