College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China.
College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China.
Bioresour Technol. 2020 Jan;295:121997. doi: 10.1016/j.biortech.2019.121997. Epub 2019 Sep 6.
Swine manure is considered a reservoir for antibiotic resistance genes (ARGs), which may enter the soil and then the food chain to endanger human health. This study investigated the effects of adding 0%, 5%, and 15% (w/w) macroporous adsorption resin (MAR) on ARGs and the bacterial community during composting. The results showed that the addition of MAR reduced the abundances of ARGs (14.14-99.44%) and mobile genetic elements (MGEs) (47.83-99.48%) after swine manure composting. Significant positive correlations were detected between ARGs and MGEs, and thus the variations in MGEs may have led to the changes in ARGs. Redundancy analysis showed that MGEs had stronger effects on ARGs than environmental factors and the bacterial community. Network analysis suggested that ARGs and MGEs co-existed in common host bacteria. In conclusion, the results showed that adding 5% MAR can reduce the risk of ARG transmission.
猪粪被认为是抗生素抗性基因（ARGs）的储存库，这些基因可能进入土壤，然后进入食物链，危害人类健康。本研究调查了在堆肥过程中添加 0%、5%和 15%（w/w）大孔吸附树脂（MAR）对 ARGs 和细菌群落的影响。结果表明，MAR 的添加降低了猪粪堆肥后 ARGs（14.14-99.44%）和移动遗传元件（MGEs）（47.83-99.48%）的丰度。ARGs 与 MGEs 之间存在显著的正相关关系，因此 MGEs 的变化可能导致了 ARGs 的变化。冗余分析表明，MGEs 对 ARGs 的影响大于环境因素和细菌群落。网络分析表明，ARGs 和 MGEs 共同存在于常见的宿主细菌中。总之，研究结果表明，添加 5%的 MAR 可以降低 ARG 传播的风险。
