Department of Clinical Laboratory, Fudan University Shanghai Cancer Center, 270 DongAn Road, Xuhui District, Shanghai, 200032, China.
Department of Clinical Laboratory, Shanghai Proton and Heavy Ion Center, Shanghai, 201321, China.
BMC Biochem. 2018 Jul 31;19(1):6. doi: 10.1186/s12858-018-0096-6.
Endonucleases play critical roles in maintaining genomic stability and regulating cell growth. The purpose of this study was to evaluate detection of endonuclease activity as an indicator in the early diagnosis and prognosis of lymphoma.
The method of endonuclease activity determination was successfully established and applied to compare cancer patient and control cohorts. Endonuclease activities of cancer tissues were significantly higher than those of adjacent control tissues (P < 0.001). We next investigated endonuclease activity in peripheral blood of enrolled patients and the controls, which were also significantly higher in patients than in controls (P = 0.015). Additionally, endonuclease activities were elevated in the metastasis subgroup compared with the non-metastasis subgroup(P = 0.038), whereas no significant difference was found between age(≤ 56y, > 56y) and gender (P = 0.736 > 0.05 and P = 0.635 > 0.05, respectively). Although there was no significant difference between control group with the non-metastatic cancer patients (P = 0.800 > 0.05), endonuclease activities were lower in the control group compared with the non-metastatic cancer patients with lymphoma (P = 0.033). The progression-free survival probability of patients with elevated R ratios(R ratio ≥ 1.4) was significantly lower than that of patients with lower R ratios (R ratio < 1.4).
An assay was established to detect the endonuclease activity,which might be useful for the prognosis of cancers, especially lymphoma.
内切核酸酶在维持基因组稳定性和调节细胞生长方面起着关键作用。本研究旨在评估内切核酸酶活性作为淋巴瘤早期诊断和预后的指标。
成功建立了内切核酸酶活性测定方法，并应用于比较癌症患者和对照队列。癌症组织的内切核酸酶活性明显高于相邻对照组织（P<0.001）。我们接下来研究了入组患者和对照组的外周血内切核酸酶活性，患者组明显高于对照组（P=0.015）。此外，转移亚组的内切核酸酶活性高于非转移亚组（P=0.038），而年龄（≤56y，>56y）和性别（P=0.736>0.05 和 P=0.635>0.05）之间无显著差异。虽然对照组与非转移性癌症患者之间无显著差异（P=0.800>0.05），但对照组的内切核酸酶活性低于非转移性淋巴瘤癌症患者（P=0.033）。R 比值（R ratio≥1.4）升高的患者无进展生存概率明显低于 R 比值较低的患者（R ratio<1.4）。
建立了一种检测内切核酸酶活性的方法，该方法可能对癌症，特别是淋巴瘤的预后有用。
