Deng Yue, Deng Chunping, Yang Jinshui, Li Baozhen, Wang Entao, Yuan Hongli
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.
Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico.
Front Microbiol. 2018 Jul 17;9:1576. doi: 10.3389/fmicb.2018.01576. eCollection 2018.
To investigate the diversity of butane-oxidizing bacteria in soils contaminated by long-term light hydrocarbon microseepage and the influence of butane on the soil microbial community, a quantitative study and identification of butane-oxidizing bacteria (BOB) in soils at the Puguang gas field were performed by DNA-based stable isotope probing (DNA-SIP). For the first time, two phylotypes corresponding to the genera and were identified as being directly involved in butane oxidation, in addition to the well-known light hydrocarbon degrader . Furthermore, genes were strongly labeled by C-butane, and their abundances in gas field soils increased by 43.14-, 17.39-, 21.74-, and 30.14-fold when incubated with butane for 6, 9, 12, and 14 days, respectively, indicating that these harboring bacteria could use butane as the sole carbon and energy source and they play an important role in butane degradation. We also found that the addition of butane rapidly shaped the bacterial community and reduced the diversity of genes in the gas field soils. These findings improve our understanding of BOB in the gas field environment and reveal the potential for their applications in petroleum exploration and bioremediation.
为了研究长期轻质烃微渗漏污染土壤中丁烷氧化细菌的多样性以及丁烷对土壤微生物群落的影响，通过基于DNA的稳定同位素探测（DNA-SIP）对普光气田土壤中的丁烷氧化细菌（BOB）进行了定量研究和鉴定。除了众所周知的轻质烃降解菌外，首次鉴定出与属和属相对应的两种系统发育型直接参与丁烷氧化。此外，基因被C-丁烷强烈标记，当分别与丁烷孵育6、9、12和14天时，它们在气田土壤中的丰度分别增加了43.14倍、17.39倍、21.74倍和30.14倍，这表明这些携带细菌可以利用丁烷作为唯一的碳源和能源，并且它们在丁烷降解中发挥着重要作用。我们还发现，丁烷的添加迅速塑造了细菌群落，并降低了气田土壤中基因的多样性。这些发现增进了我们对气田环境中BOB的理解，并揭示了它们在石油勘探和生物修复中的应用潜力。
