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GmMYB181，一种大豆R2R3-MYB蛋白，增加转基因植株的分枝数。

GmMYB181, a Soybean R2R3-MYB Protein, Increases Branch Number in Transgenic .

作者信息

Yang Hui, Xue Qian, Zhang Zhenzhen, Du Jingyi, Yu Deyue, Huang Fang

机构信息

National Center for Soybean Improvement, Key Laboratory of Biology and Genetic Improvement of Soybean Ministry of Agriculture, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, China.

出版信息

Front Plant Sci. 2018 Jul 17;9:1027. doi: 10.3389/fpls.2018.01027. eCollection 2018.

DOI:10.3389/fpls.2018.01027
PMID:30065741
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6056663/
Abstract

Soybean () is an important economic crop that provides abundant oil and high quality protein for human beings. As the process of reproductive growth directly determines the crop seed yield and quality, we initiated studies to identify genes that regulate soybean floral organ development. One R2R3-MYB transcription factor gene, designated as , was found to be enriched in flowers based on microarray analysis and was further functionally investigated in transgenic . GmMYB181 protein contains two MYB domains, which localized to the nucleus and displayed transcriptional activation in yeast hybrid system. Real-time quantitative PCR (qRT-PCR) results suggested exclusively expressed in flower tissue. In , overexpression of altered the morphology of floral organs, fruit size and plant architecture, including outward curly sepals, smaller siliques, increased lateral branches and reduced plant height, indicating that is involved in the development of reproductive organs and plays an important role in controlling plant architecture. Further, microarray analysis revealed that overexpressing in affected the expression of 3450 genes in mature flowers, including those involved in floral organ, seed/fruit development, and responded to different hormone signals.

摘要

大豆（）是一种重要的经济作物，为人类提供丰富的油脂和优质蛋白质。由于生殖生长过程直接决定作物种子的产量和品质，我们开展了研究以鉴定调控大豆花器官发育的基因。基于微阵列分析发现一个R2R3-MYB转录因子基因，命名为，在花中表达量富集，并在转基因中对其进行了进一步的功能研究。GmMYB181蛋白含有两个MYB结构域，定位于细胞核，并在酵母杂交系统中表现出转录激活活性。实时定量PCR（qRT-PCR）结果表明在花组织中特异性表达。在中，的过表达改变了花器官形态、果实大小和植株结构，包括萼片向外卷曲、角果变小、侧枝增多和株高降低，表明参与生殖器官的发育并在控制植株结构中发挥重要作用。此外，微阵列分析显示在中过表达影响成熟花中3450个基因的表达，包括那些参与花器官、种子/果实发育的基因，并对不同激素信号作出响应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9f/6056663/4f2d4d9ebaf9/fpls-09-01027-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9f/6056663/6c24747cbdf7/fpls-09-01027-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9f/6056663/c4790a2eac3a/fpls-09-01027-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9f/6056663/86c2eadc4a61/fpls-09-01027-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9f/6056663/df2d811625bd/fpls-09-01027-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9f/6056663/a219f5ee0ce4/fpls-09-01027-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9f/6056663/3c5a175f337f/fpls-09-01027-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9f/6056663/a39b3ff8834e/fpls-09-01027-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9f/6056663/13a7a5750de9/fpls-09-01027-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9f/6056663/4f2d4d9ebaf9/fpls-09-01027-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9f/6056663/6c24747cbdf7/fpls-09-01027-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9f/6056663/c4790a2eac3a/fpls-09-01027-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9f/6056663/86c2eadc4a61/fpls-09-01027-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9f/6056663/df2d811625bd/fpls-09-01027-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9f/6056663/a219f5ee0ce4/fpls-09-01027-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9f/6056663/3c5a175f337f/fpls-09-01027-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9f/6056663/a39b3ff8834e/fpls-09-01027-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9f/6056663/13a7a5750de9/fpls-09-01027-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ca9f/6056663/4f2d4d9ebaf9/fpls-09-01027-g009.jpg

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