Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, P. R. China.
Shanghai Suchuang Diagnostic Products Co., Ltd, Shanghai 201318, P. R. China and Shanghai Suxin Biotechnology Co. Ltd, Shanghai 201318, P. R. China.
Chem Commun (Camb). 2018 Sep 25;54(75):10562-10565. doi: 10.1039/c8cc04577a. Epub 2018 Aug 1.
Here, we introduced a novel exonuclease-assisted isothermal nucleic acid amplification (Exo-NAT) utilizing the full-length Bst DNA polymerase combined with a melting curve analysis. This method achieved an ultrahigh specificity with a good detection limit (10 copies) in both singleplex and multiplex detection, which was validated by detecting three diarrhea-inducing pathogens, rotavirus A, astrovirus, and adenovirus, in 42 clinical samples.
在这里，我们介绍了一种新型的外切酶辅助等温核酸扩增（Exo-NAT）技术，该技术利用全长 Bst DNA 聚合酶结合熔解曲线分析。该方法在单重和多重检测中均具有超高的特异性和良好的检测限（10 拷贝），在 42 份临床样本中检测轮状病毒 A、星状病毒和腺病毒这三种致腹泻病原体时得到了验证。
