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用于即时护理时进行定性、定量和数字核酸检测的全集成微流控设备。

Fully integrated microfluidic devices for qualitative, quantitative and digital nucleic acids testing at point of care.

作者信息

Li Zedong, Bai Yuemeng, You Minli, Hu Jie, Yao Chunyan, Cao Lei, Xu Feng

机构信息

The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Xi'an Jiaotong University, Xi'an, 710049, PR China; Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University, Xi'an, 710049, PR China.

Suzhou DiYinAn Biotechnology Co., Ltd, Suzhou, 215010, PR China.

出版信息

Biosens Bioelectron. 2021 Apr 1;177:112952. doi: 10.1016/j.bios.2020.112952. Epub 2020 Dec 31.

DOI:10.1016/j.bios.2020.112952
PMID:33453463
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7774487/
Abstract

Benefiting from emerging miniaturized and equipment-free nucleic acid testing (NAT) technologies, fully integrated NAT devices at point of care (POC) with the capability of "sample-in-answer-out" are proceeding at a break-neck speed to eliminate complex operations and reduce the risk of contamination. Like the development of polymerase chain reaction (PCR) technology (the standard technique for NAT), the detection signal of fully integrated NAT devices has evolved from qualitative to quantitative and recently to digital readout, aiming at expanding their extensive applications through gradually improving detection sensitivity and accuracy. This review firstly introduces the existing commercial products, and then illustrates recent fully integrated microfluidic devices for NAT at POC from the aspect of detection signals (i.e., qualitative, quantitative and digital). Importantly, the key issues of existing commercial products and the main challenges between scientific research and product development are discussed. On this basis, we envision that the MARCHED (miniaturized, automatic, reagent-preloaded, commercializable, high-throughput, environment-independent and disposable) NAT devices are expected to be realized in the near future.

摘要

受益于新兴的小型化且无需设备的核酸检测（NAT）技术，具备“样本进结果出”能力的即时检测（POC）全集成NAT设备正以极快的速度发展，以消除复杂操作并降低污染风险。与聚合酶链反应（PCR）技术（NAT的标准技术）的发展类似，全集成NAT设备的检测信号已从定性发展到定量，最近又发展到数字读出，旨在通过逐步提高检测灵敏度和准确性来拓展其广泛应用。本文首先介绍现有的商业产品，然后从检测信号（即定性、定量和数字）方面阐述近期用于POC的全集成微流控NAT设备。重要的是，讨论了现有商业产品的关键问题以及科研与产品开发之间的主要挑战。在此基础上，我们设想在不久的将来有望实现小型化、自动化、预装试剂、可商业化、高通量、不受环境影响且一次性使用（MARCHED）的NAT设备。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c040/7774487/2b4aefa88645/gr6_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c040/7774487/c05f761f1264/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c040/7774487/939475788a84/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c040/7774487/02f2c7d33658/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c040/7774487/3837ccf600f9/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c040/7774487/bd6b14f2ab81/gr5_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c040/7774487/2b4aefa88645/gr6_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c040/7774487/c05f761f1264/gr1_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c040/7774487/939475788a84/gr2_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c040/7774487/02f2c7d33658/gr3_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c040/7774487/3837ccf600f9/gr4_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c040/7774487/bd6b14f2ab81/gr5_lrg.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c040/7774487/2b4aefa88645/gr6_lrg.jpg

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