Baldini G, Passamonti S, Lunazzi G C, Tiribelli C, Sottocasa G L
Biochim Biophys Acta. 1986 Mar 27;856(1):1-10. doi: 10.1016/0005-2736(86)90002-7.
The movement of sulfobromophthalein is measured in rat liver plasma-membrane vesicles by direct dual-wavelength spectrophotometry. The technique is based on the principle that the dye, when entering a more acidic compartment, changes its absorption in the visible region. From this study it may be concluded that, among the different cellular subfractions, only liver plasma-membrane vesicles can catalyze electrogenic transport of sulfobromophthalein. Plasma membranes from erythrocytes are unable to perform such a function. The movement follows the distribution pattern of (Na+ + K+)-ATPase and it is therefore concluded that this process occurs exclusively at the sinusoidal membrane level. Inhibition studies confirm that the process is catalyzed by bilitranslocase.
通过直接双波长分光光度法测定大鼠肝血浆膜囊泡中磺溴酞的转运。该技术基于这样一个原理：染料进入酸性更强的区室时，其在可见光区域的吸收会发生变化。从这项研究可以得出结论，在不同的细胞亚组分中，只有肝血浆膜囊泡能够催化磺溴酞的电转运。红细胞的质膜无法执行这种功能。该转运遵循（Na⁺ + K⁺）-ATP酶分布模式，因此得出结论，该过程仅发生在肝血窦膜水平。抑制研究证实该过程由胆盐转运蛋白催化。
