Herak J N, Udovicić L, Pifat G, Brnjas-Kraljević J, Jürgens G, Holasek A
Biochim Biophys Acta. 1986 Apr 15;876(2):200-9. doi: 10.1016/0005-2760(86)90275-4.
The ESR technique has been used to study binding of Mn(II) ions to low-density lipoprotein (LDL) in solutions of various electrolyte ionic strengths. A model of the binding has been proposed which describes all the observations in electrolytes of ten different concentrations in terms of two types of binding sites and two corresponding sets of intrinsic binding parameters (n1 = 8, Kd1 = 1.31 X 10(-3) mol X l-1 and n2 = 170, Kd2 = 5.71 X 10(-2) mol X l-1). These parameters, together with the values of the potential (phi 0) responsible for binding of the ions to specific charged sites on the surface, reproduce the observed binding curves well in all the systems studied. The phi 0 values are obtained as an appropriate solution of the Poisson-Boltzmann equation.
电子自旋共振（ESR）技术已被用于研究在各种电解质离子强度的溶液中，锰（II）离子与低密度脂蛋白（LDL）的结合。已经提出了一种结合模型，该模型用两种类型的结合位点和两组相应的固有结合参数（n1 = 8，Kd1 = 1.31×10^(-3) mol·L^(-1) 和 n2 = 170，Kd2 = 5.71×10^(-2) mol·L^(-1)）描述了十种不同浓度电解质中的所有观测结果。这些参数，连同负责离子与表面特定带电位点结合的电位（phi 0）值，在所有研究的系统中都能很好地重现观察到的结合曲线。phi 0值是作为泊松 - 玻尔兹曼方程的一个合适解得到的。
