Autillo-Touati A, Mallat M, Araud D, Moura Neto V, Vuillet J, Glowinski J, Seite R, Prochiantz A
Brain Res. 1986 Apr;391(1):33-47. doi: 10.1016/0165-3806(86)90005-2.
In two preceding papers we described the cloning of two astrocytic cell lines by simian virus 40 (SV40) transformation of embryonic mouse mesencephalon (F7-Mes) and striatum (F12-Str). The characterization of these lines as belonging to the astrocytic lineage is based on pharmacological, immunocytochemical and physiological data. Here we present quantitative and qualitative data on the morphological aspects of these two astrocytic clones observed under light and electron microscopy. We show that the clones present ultrastructural characters reminiscent of the morphology of young astrocytes. On one hand, they are rather similar to primary astrocytes in culture; on the other, they differ both from a clonal fibroblastic cell line (BT2) and from embryonic mouse fibroblasts in primary culture. These astroblastic clones display 4 morphologically different cell populations which we called types I, II, III and IV. Types II and III are very similar and represent the most predominant cells; their morphologies strongly remind of that of astroblasts. Type I corresponds to glioblasts and does not account for more than 15-20% of the total population. Type IV, which is very similar to differentiated velamentous astrocytes, normally represent ca. 5% of the cells. However, when the transformed cells are treated with mitomycin or mitomycin + dibutyryl cyclic AMP (dbcAMP), the proportion of type IV cells increases very much (up to more than 50% of the cells) while types I, II and III become less numerous. Morphological analysis therefore confirms that the two cell lines derived from the SV40 transformation of 14-day-old embryonic mesencephalic and striatal cells belong to the astrocytic lineage. Moreover, it seems that they can differentiate in vitro in cell culture conditions either spontaneously or under the action of pharmacological treatments known to enhance normal astrocyte maturation.
在之前的两篇论文中，我们描述了通过猿猴病毒40（SV40）转化胚胎小鼠中脑（F7-Mes）和纹状体（F12-Str）克隆出两种星形胶质细胞系。将这些细胞系归为星形胶质细胞谱系的特征描述基于药理学、免疫细胞化学和生理学数据。在此，我们呈现关于这两种星形胶质细胞克隆在光学显微镜和电子显微镜下观察到的形态学方面的定量和定性数据。我们表明，这些克隆呈现出超微结构特征，让人联想到年轻星形胶质细胞的形态。一方面，它们与培养中的原代星形胶质细胞相当相似；另一方面，它们既不同于克隆性成纤维细胞系（BT2），也不同于原代培养的胚胎小鼠成纤维细胞。这些星形母细胞克隆显示出4种形态不同的细胞群体，我们将其称为I型、II型、III型和IV型。II型和III型非常相似，是最主要的细胞类型；它们的形态强烈让人联想到星形母细胞。I型对应于成神经胶质细胞，在总数中占比不超过15 - 20%。IV型与分化的帆状星形胶质细胞非常相似，通常占细胞总数的约5%。然而，当用丝裂霉素或丝裂霉素 + 二丁酰环磷腺苷（dbcAMP）处理转化细胞时，IV型细胞的比例会大幅增加（高达细胞总数的50%以上），而I型、II型和III型细胞则变得较少。因此，形态学分析证实，源自14日龄胚胎中脑和纹状体细胞的SV40转化的这两种细胞系属于星形胶质细胞谱系。此外，似乎它们在细胞培养条件下能够自发地或在已知能促进正常星形胶质细胞成熟的药理学处理作用下进行体外分化。
