Autillo-Touati A, Chamak B, Araud D, Vuillet J, Seite R, Prochiantz A
Laboratoire de Biologie Cellulaire et Tissulaire, Faculté de Médecine, Marseille, France.
J Neurosci Res. 1988 Mar;19(3):326-42. doi: 10.1002/jnr.490190308.
Mesencephalic neurons were cultured for 2 days on mesencephalic or striatal astrocyte monolayers. The morphology of these neurons was studied in electron microscopy. The number of dendritic profiles was higher on mesencephalic astrocytes (homotopic neuro-astroglial co-cultures) than on striatal astrocytes (heterotopic co-cultures). This increase in the number of dendrites correlated with a more mature aspect of the neurons. Striatal neurons were also cultured on the astrocytic monolayers. The state of maturation of these neurons was more advanced, and the number of their dendrites was higher on striatal than on mesencephalic astrocytes. These results confirm and extend the fact that neuronal maturation and dendritic growth can be regulated through region-specific neuro-astroglial interactions (Denis-Donini et al., 1984; Chamak et al., 1987).
中脑神经元在中脑或纹状体星形胶质细胞单层上培养2天。通过电子显微镜研究这些神经元的形态。中脑星形胶质细胞（同位神经-星形胶质细胞共培养）上的树突轮廓数量高于纹状体星形胶质细胞（异位共培养）上的数量。树突数量的增加与神经元更成熟的状态相关。纹状体神经元也在星形胶质细胞单层上培养。这些神经元的成熟状态更高级，并且它们在纹状体星形胶质细胞上的树突数量高于中脑星形胶质细胞上的数量。这些结果证实并扩展了神经元成熟和树突生长可通过区域特异性神经-星形胶质细胞相互作用来调节这一事实（Denis-Donini等人，1984年；Chamak等人，1987年）。
