Department of Toxicology, Wroclaw Medical University, Wroclaw, Poland.
Department of Urology and Oncological Urology, Wroclaw Medical University, Wroclaw, Poland.
Biomed Res Int. 2018 Jul 5;2018:8315410. doi: 10.1155/2018/8315410. eCollection 2018.
Uroplakins are glycoproteins investigated as potential markers of urothelial carcinoma. However, their role in chemical carcinogenesis is uncertain. In this study the diagnostic value of plasma and urine uroplakin IIIa (UPIIIa) levels in bladder cancer (BC) was investigated, particularly in the aspect of environmental exposure to chemical carcinogens, measured by DNA damage and detoxification ability in the BC smoking group. The correlation between uroplakin, 8-OHdG, and GST was investigated.
This study included 61 BC patients and 33 healthy controls. UPIIIa, 8-OHdG, and GST levels were estimated by the immunoenzymatic method (ELISA).
UPIIIa levels were elevated in BC patients in plasma (p≤0.001) and in urine (p≤0.001), as were 8-OHdG and GST levels in urine. Moreover, the 8-OHdG level was higher in invasive or high grade tumors. A positive correlation between UPIIIa/GST and 8-OHdG/GST was observed, but no UPIIIa/8-OHdG correlation was noted.
The study showed the diagnostic value of urine and plasma UPIIIa in BC (good sensitivity, specificity, and predictive value). The lack of UPIIIa correlation with 8-OHdG and smoking suggests that UPIIIa does not reflect the environmental exposure. The increased levels of 8-OHdG and GST in the invasive tumor stage indicate their value in BC monitoring.
尿路上皮蛋白被认为是潜在的膀胱癌标志物。然而，其在化学致癌作用中的作用尚不清楚。本研究旨在探讨尿路上皮蛋白 IIIa（UPIIIa）在膀胱癌（BC）患者血浆和尿液中的诊断价值，特别是在 BC 吸烟组中，通过 DNA 损伤和解毒能力来检测环境中化学致癌物的暴露情况。同时还研究了尿路上皮蛋白与 8-OHdG 和 GST 的相关性。
本研究共纳入 61 例 BC 患者和 33 例健康对照者。采用酶联免疫吸附试验（ELISA）检测 UPIIIa、8-OHdG 和 GST 水平。
BC 患者血浆和尿液中 UPIIIa 水平均升高（p≤0.001），尿液中 8-OHdG 和 GST 水平也升高。此外，侵袭性或高级别肿瘤患者的 8-OHdG 水平更高。UPIIIa/GST 与 8-OHdG/GST 之间呈正相关，但 UPIIIa 与 8-OHdG 之间无相关性。
本研究表明尿液和血浆 UPIIIa 对 BC 具有诊断价值（具有良好的敏感性、特异性和预测价值）。UPIIIa 与 8-OHdG 和吸烟之间缺乏相关性提示其不能反映环境暴露情况。侵袭性肿瘤阶段 8-OHdG 和 GST 水平的升高表明其在 BC 监测中的价值。
