Shannon B T, Roach J, Cheek-Luten M, Ruymann F B
Diagn Immunol. 1986;4(1):37-42.
Children with hemophilia A are at risk for the acquired immunodeficiency syndrome (AIDS). Clinically asymptomatic hemophiliacs demonstrate many immune abnormalities that might represent exposure to the AIDS agent through blood products or be a natural reaction to their therapy. In this study, we examined lymphocyte subset distribution in children with hemophilia A who had been exposed to HTLV-III as determined by antibody seroconversion. Seroconversion to HTLV-III was confirmed using Western blot analysis. The lymphocyte subsets studied included T4+ and T8+ cells. The distribution of lymphocyte subsets in children with hemophilia A was independent of seroconversion to HTLV-III. Children with hemophilia A treated with commercial factor VIII concentrate had normal numbers of circulating T4+ lymphocytes and significantly increased numbers of circulating T8+ lymphocytes compared with their nontransfused age-matched counterparts. An increased number of T8+ lymphocytes was not observed, however, in children treated exclusively with cryoprecipitate. These results suggest that HTLV-III alone cannot account for changes in lymphocyte subsets in hemophiliacs. Higher antigenic protein loads in factor VIII concentrate or additional factors might account for the increased absolute numbers of T8+ lymphocytes and represent a natural response to therapy.
患有甲型血友病的儿童有感染获得性免疫缺陷综合征（艾滋病）的风险。临床上无症状的血友病患者表现出许多免疫异常，这些异常可能代表通过血液制品接触了艾滋病病原体，或者是对其治疗的自然反应。在本研究中，我们检查了经抗体血清转化确定已接触人嗜T淋巴细胞病毒III型（HTLV-III）的甲型血友病儿童的淋巴细胞亚群分布。使用蛋白质印迹分析确认了对HTLV-III的血清转化。所研究的淋巴细胞亚群包括T4+和T8+细胞。甲型血友病儿童的淋巴细胞亚群分布与对HTLV-III的血清转化无关。与未输血的年龄匹配儿童相比，接受商业性凝血因子VIII浓缩物治疗的甲型血友病儿童循环T4+淋巴细胞数量正常，循环T8+淋巴细胞数量显著增加。然而，仅接受冷沉淀治疗的儿童未观察到T8+淋巴细胞数量增加。这些结果表明，仅HTLV-III不能解释血友病患者淋巴细胞亚群的变化。凝血因子VIII浓缩物中较高的抗原蛋白负荷或其他因素可能解释了T8+淋巴细胞绝对数量的增加，并代表了对治疗的自然反应。
