Dey Kishore K, Leite Milena, Hu John S, Jordan Ramon, Melzer Michael J
Department of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, HI, 96822, USA.
Division of Plant Industry, Florida Department of Agriculture and Consumer Services, Gainesville, FL, 32614, USA.
Arch Virol. 2018 Nov;163(11):3051-3058. doi: 10.1007/s00705-018-3947-y. Epub 2018 Aug 1.
Star jasmine (Jasminum multiflorum) plants growing in Hawaii expressing a diverse array of virus-like foliar symptoms were examined for the presence of a causal agent. Symptomatic tissues collected from three locations on the island of Oahu, Hawaii consistently harbored double-stranded (ds)RNAs approximately 4.2 and 1.7 kbp in size. Sanger and high-throughput sequencing approaches revealed these dsRNAs were from two distinct virus species co-infecting the same host plant. One of these two viruses was the recently characterized Jasmine virus H (JaVH), and the second we designated as Jasmine mosaic-associated virus (JMaV). Both viruses were subsequently found, by high-throughput sequencing, in a single angelwing jasmine (J. nitidum) plant exhibiting similar ringspot symptoms and growing at the U.S. National Arboretum in Washington, DC. Phylogenetic placement, genome organization, and sequence comparisons indicate these two viruses are classifiable as members of the genus Pelarspovirus (family Tombusviridae). To determine if either of these viruses were associated with the observed symptoms, a PCR-based detection assay was developed to detect and distinguish these two viruses in several Hawaii-grown plants. All 32 samples collected from four Oahu locations displayed symptoms. All 32 samples were positive for JaVH, and 16 were positive for JMaV. An asymptomatic star jasmine plant from the island of Hawaii was negative for both JaVH and JMaV. Both viruses were also found in a symptomatic J. sambac sample from Maryland while only JMaV was detected in a symptomatic Jasminum sp. sample from California.
对生长在夏威夷、表现出多种类病毒叶部症状的星茉莉（多花素馨）植株进行了病原体检测。从夏威夷瓦胡岛三个地点采集的有症状组织中，始终存在大小约为4.2千碱基对（kbp）和1.7 kbp的双链（ds）RNA。桑格测序法和高通量测序法显示，这些dsRNA来自两种不同的病毒，它们共同感染同一宿主植物。这两种病毒中的一种是最近鉴定出的茉莉病毒H（JaVH），另一种我们命名为茉莉花叶相关病毒（JMaV）。随后，通过高通量测序，在华盛顿特区美国国家植物园一株表现出类似环斑症状的单叶天使茉莉（亮叶素馨）植株中发现了这两种病毒。系统发育定位、基因组结构和序列比较表明，这两种病毒可归类为番茄丛矮病毒科（Tombusviridae）番茄丛矮病毒属（Pelarspovirus）的成员。为了确定这两种病毒是否与观察到的症状有关，开发了一种基于聚合酶链反应（PCR）的检测方法，以检测和区分几种在夏威夷种植的植物中的这两种病毒。从瓦胡岛四个地点采集的所有32个样本都表现出症状。所有32个样本的JaVH检测均为阳性，16个样本的JMaV检测为阳性。来自夏威夷岛的一株无症状星茉莉植株的JaVH和JMaV检测均为阴性。在一份来自马里兰州的有症状的 sambac 样本中也发现了这两种病毒，而在一份来自加利福尼亚州的有症状的素馨属样本中仅检测到JMaV。
