State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, 610041, China.
Department of Conservation Dentistry and Endodontics, Stomatological Hospital of Chongqing Medical University, Chongqing, 401147, China.
Curr Med Sci. 2018 Feb;38(1):160-166. doi: 10.1007/s11596-018-1860-y. Epub 2018 Mar 15.
This study aimed to study whether the Sortase A (srtA) gene helps mediate coaggregation and co-adherence between Streptococcus mutans (S. mutans) and other salivary bacteria. S. mutans UA159 and srtA-deficient mutant served as "bait" in classical co-aggregation assays and membrane-based co-adherence assays were used to examine interactions of S. mutans with Fusobacterium nucleatum (F. nucleatum), Streptococcus mitis (S. mitis), Streptococcus gordonii (S. gordonii), Streptococcus sanguis (S. sanguis), Actinomyces naeslundii (A. naeslundii) and Lactobacillus. Co-adherence assays were also performed using unfractionated saliva from healthy individuals. Co-adhering partners of S. mutans were sensitively detected using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Both UA159 and its srtA-deficient mutant bound to F. nucleatum but not to any of the other five salivary bacteria. The srtA-deficient mutant showed lower co-adherence with F.nucleatum. The two S. mutans strains also showed similar co-adherence profiles against unfractionated salivary bacteria, except that UA159 S. mutans but not the srtA-deficient bound to a Neisseria sp. under the same conditions. Deleting srtA reduces the ability of S. mutans to bind to F.nucleatum, but it does not appear to significantly affect the binding profile of S. mutans to bulk salivary bacteria.
本研究旨在研究唾液链球菌(S. mutans)的 Sortase A (srtA) 基因是否有助于介导其与其他口腔细菌的共聚和共附着。S. mutans UA159 和 srtA 缺陷突变体被用作经典共聚实验中的“诱饵”，而膜基共附着实验则用于检测 S. mutans 与核梭杆菌(F. nucleatum)、缓症链球菌(S. mitis)、戈登链球菌(S. gordonii)、血链球菌(S. sanguis)、内氏放线菌(A. naeslundii)和嗜酸乳杆菌(Lactobacillus)的相互作用。还使用来自健康个体的未分级唾液进行了共附着实验。使用聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）灵敏地检测了 S. mutans 的共附着伙伴。UA159 及其 srtA 缺陷突变体均与 F. nucleatum 结合，但与其他五种唾液细菌均不结合。srtA 缺陷突变体与 F.nucleatum 的共附着能力较低。两种 S. mutans 菌株对未分级唾液细菌也表现出相似的共附着谱，只是在相同条件下，UA159 S. mutans 而不是 srtA 缺陷突变体与一种奈瑟菌属结合。删除 srtA 会降低 S. mutans 与 F.nucleatum 结合的能力，但似乎不会显著影响 S. mutans 与大量唾液细菌的结合谱。
