Department of Preventive Dentistry, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China.
Arch Oral Biol. 2013 Sep;58(9):1129-38. doi: 10.1016/j.archoralbio.2013.04.005. Epub 2013 May 9.
To monitor the longitudinal changes in oral microbial diversity of children with severe early childhood caries (S-ECC) compared to caries free (CF) controls.
Dental plaque samples of 12 children in each group at 8, 14, 20, 26 and 32 months of age were analysed. Total microbial genomic DNA was isolated from each sample, and PCR-denaturing gradient gel electrophoresis (DGGE) analyses were carried out.
The number of bands was significantly higher in the CF group (18.17±4.91 bands) than in the S-ECC group (14.54±5.56 bands) at 32 months of age (P<0.05). A total of 21 genera were identified in all subjects, and there were no significant differences between the two groups at genus level. DGGE profiles showed that most of the clusters were constructed from one individual over time in the both groups.
The onset of S-ECC is accompanied by a decrease in microbial diversity. The overall composition of the microbiota is highly similar within an individual over time.
监测严重婴幼儿龋（S-ECC）患儿与无龋（CF）对照组儿童口腔微生物多样性的纵向变化。
分别于 8、14、20、26 和 32 月龄时采集每组 12 名儿童的牙菌斑样本。从每个样本中提取总微生物基因组 DNA，并进行 PCR-变性梯度凝胶电泳（DGGE）分析。
32 月龄时，CF 组的条带数明显高于 S-ECC 组（18.17±4.91 条带比 14.54±5.56 条带）（P<0.05）。在所有受试者中总共鉴定出 21 个属，两组之间在属水平上没有显著差异。DGGE 图谱显示，两组中大多数聚类都是随着时间的推移由一个个体构成的。
S-ECC 的发生伴随着微生物多样性的减少。个体内的微生物群组成在时间上高度相似。
