Divers M, Craven P L, Vivian A
J Gen Microbiol. 1985 Dec;131(12):3367-74. doi: 10.1099/00221287-131-12-3367.
The non-conjugative plasmid pAV5 specifies resistance to kanamycin/neomycin (KmR) and tetracycline (TcR). Physical evidence is presented to show that pAV5 gives rise to two plasmids, pAV51 (KmR) and pAV52 (TcR), which are formed by deletion of apparently non-overlapping segments of pAV5. Expression of TcR has been obtained in Escherichia coli and is associated with a 1.9 kb HindIII fragment found in pAV5 and in pAV52. Expression of KmR has been obtained in E. coli and is associated with a 1.3 kb PstI fragment found in pAV5 and pAV51. Evidence is presented that the KmR gene is flanked by inverted repeat sequences and is therefore tentatively identified as a transposon, designated Tn4411. The KmR gene specifies an aminoglycoside 3'-phosphotransferase-type I (APH(3')-I) enzyme.
非接合性质粒pAV5赋予对卡那霉素/新霉素（KmR）和四环素（TcR）的抗性。有物理证据表明，pAV5产生了两种质粒，pAV51（KmR）和pAV52（TcR），它们是通过缺失pAV5明显不重叠的片段形成的。在大肠杆菌中获得了TcR的表达，并且与在pAV5和pAV52中发现的1.9 kb HindIII片段相关。在大肠杆菌中获得了KmR的表达，并且与在pAV5和pAV51中发现的1.3 kb PstI片段相关。有证据表明，KmR基因两侧是反向重复序列，因此初步鉴定为转座子，命名为Tn4411。KmR基因编码一种I型氨基糖苷3'-磷酸转移酶（APH(3')-I）酶。
