对卡那霉素具有抗性的载体，它们是质粒pUC8、pUC9、pEMBL8和pEMBL9的类似物。
Kanamycin-resistant vectors that are analogues of plasmids pUC8, pUC9, pEMBL8 and pEMBL9.
作者信息
Spratt B G, Hedge P J, te Heesen S, Edelman A, Broome-Smith J K
出版信息
Gene. 1986;41(2-3):337-42. doi: 10.1016/0378-1119(86)90117-4.
PMID:3011607
Abstract
Analogues of the cloning vectors pUC8, pUC9, pEMBL8 +/- and pEMBL9 +/- that have kanamycin resistance (KmR) instead of ampicillin resistance (ApR) as the selectable marker have been developed. HindIII and SmaI sites within the KmR gene have been removed so that all of the cloning sites in the multi-linker region of these plasmids may be used except the AccI site.
摘要
已经开发出了克隆载体pUC8、pUC9、pEMBL8 +/-和pEMBL9 +/-的类似物，这些类似物具有卡那霉素抗性（KmR）而非氨苄青霉素抗性（ApR）作为选择标记。KmR基因内的HindIII和SmaI位点已被去除，以便这些质粒多接头区域中的所有克隆位点（AccI位点除外）都可以使用。
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