Hammer S M, Gillis J M
J Biol Response Mod. 1986 Feb;5(1):36-44.
The effects of recombinant interleukin-2 on resting T lymphocytes were examined in vitro. T cell enriched populations were isolated from the peripheral venous blood of three normal individuals by Ficoll-Hypaque gradient separation and standard sheep erythrocyte rosetting techniques. Interleukin-2 at concentrations of 100 and 1,000 U/ml stimulated tritiated thymidine incorporation, Tac antigen expression, interferon production, and blast transformation in T cell cultures. These changes were observed in the absence of cell proliferation. The effects of interleukin-2 at a concentration of 100 U/ml were substantially blocked by the addition of a 1:1,000 dilution of anti-Tac antibody to the culture medium indicating that the effects observed were mediated by the Tac receptor. These results indicate that recombinant interleukin-2 can have demonstrable functional and morphologic effects on unstimulated human T cells.
体外研究了重组白细胞介素-2对静息T淋巴细胞的作用。通过Ficoll-泛影葡胺梯度分离法和标准绵羊红细胞花环形成技术，从三名正常个体的外周静脉血中分离出富含T细胞的群体。浓度为100和1000 U/ml的白细胞介素-2刺激了T细胞培养物中氚标记胸腺嘧啶核苷的掺入、Tac抗原表达、干扰素产生和细胞增殖。在无细胞增殖的情况下观察到了这些变化。向培养基中添加1:1000稀释的抗Tac抗体可显著阻断浓度为100 U/ml的白细胞介素-2的作用，表明观察到的作用是由Tac受体介导的。这些结果表明，重组白细胞介素-2可对未受刺激的人T细胞产生明显的功能和形态学作用。
