Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, 603 203, Tamil Nadu, India.
Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, 603 203, Tamil Nadu, India.
Biochimie. 2018 Nov;154:10-18. doi: 10.1016/j.biochi.2018.07.023. Epub 2018 Aug 1.
We previously reported that ATF3 and Runx2 are involved in breast cancer progression and bone metastasis. The expression of these genes can be controlled by post-transcriptional regulators such as microRNAs (miRNAs). In this study, we identified and validated the functional role of miR-590-3p in human breast cancer cells (MDA-MB231). There was an inverse correlation between the expression of miR-590-3p and its putative target genes, ATF3 and Runx2 in these cells. Overexpression of miR-590-3p decreased the expression of ATF3 and Runx2 at the mRNA and protein levels in MDA-MB231 cells. Luciferase reporter assay identified a direct interaction of 3' UTRs of ATF3 and Runx2 with miR-590-3p in these cells. Overexpression of miR-590-3p also decreased proliferation and increased apoptosis of breast cancer cells. Based on our results, we suggest that miR-590-3p might have potential clinical applications towards controlling breast cancer progression and bone metastasis.
我们之前报道过 ATF3 和 Runx2 参与乳腺癌的进展和骨转移。这些基因的表达可以通过转录后调节因子如 microRNAs（miRNAs）来控制。在这项研究中，我们鉴定并验证了 miR-590-3p 在人乳腺癌细胞（MDA-MB231）中的功能作用。在这些细胞中，miR-590-3p 的表达与其假定的靶基因 ATF3 和 Runx2 呈负相关。miR-590-3p 的过表达降低了 MDA-MB231 细胞中 ATF3 和 Runx2 的 mRNA 和蛋白水平的表达。荧光素酶报告基因检测鉴定出 ATF3 和 Runx2 的 3'UTR 与这些细胞中的 miR-590-3p 直接相互作用。miR-590-3p 的过表达也降低了乳腺癌细胞的增殖并增加了细胞凋亡。基于我们的结果，我们认为 miR-590-3p 可能具有控制乳腺癌进展和骨转移的潜在临床应用价值。
