Abdoli R, Mirhoseini S Z, Ghavi Hossein-Zadeh N, Zamani P, Gondro C
Department of Animal Science, Faculty of Agricultural Sciences, University of Guilan, Rasht, 41996-13776, Iran.
Department of Animal Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, 65178-33131, Iran.
Anim Genet. 2018 Oct;49(5):488-491. doi: 10.1111/age.12700. Epub 2018 Aug 5.
Several causative mutations in candidate genes affecting prolificacy have been detected in various sheep breeds. A genome-wide association study was performed on estimated breeding values for litter size in Lori-Bakhtiari sheep. Prolific ewes with twinning records and others with only singleton records were genotyped using the medium-density Illumina Ovine SNP50 array. Four single nucleotide polymorphisms (SNPs) associated with litter size were identified on chromosomes 3, 6 and 22. The region on sheep chromosome 3 between 75 739 167 and 75 745 152 bp included two significant SNPs (s52383.1 and OAR3_80038014_X.1) in high linkage disequilibrium with each other. The region that surrounds these SNPs contains a novel putative candidate gene: luteinizing hormone/choriogonadotropin receptor (LHCGR), known to be involved in ovarian steroidogenesis and organism-specific biosystem pathways in sheep. Known prolificacy genes BMPR1B, BMP15 and GDF9 were not associated with litter size in Lori-Bakhtiari sheep, suggesting that other biological mechanisms could be responsible for the trait's variation in this breed.
在各种绵羊品种中已检测到影响繁殖力的候选基因中的几种致病突变。对洛里-巴赫蒂亚里羊产羔数的估计育种值进行了全基因组关联研究。使用中密度Illumina绵羊SNP50芯片对有双羔记录的多产母羊和只有单羔记录的母羊进行基因分型。在3号、6号和22号染色体上鉴定出4个与产羔数相关的单核苷酸多态性（SNP）。绵羊3号染色体上75 739 167至75 745 152 bp之间的区域包含两个彼此处于高度连锁不平衡状态的显著SNP（s52383.1和OAR3_80038014_X.1）。围绕这些SNP的区域包含一个新的假定候选基因：促黄体生成素/绒毛膜促性腺激素受体（LHCGR），已知该基因参与绵羊的卵巢类固醇生成和特定生物体生物系统途径。已知的多产基因BMPR1B、BMP15和GDF9与洛里-巴赫蒂亚里羊的产羔数无关，这表明其他生物学机制可能是该品种该性状变异的原因。
