Liu Xiujie, Liu Mengmeng, Lu Yudong, Wu Changji, Xu Yunchao, Lin Duo, Lu Dechan, Zhou Ting, Feng Shangyuan
Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Normal University, Fuzhou 350007, China.
Fujian Key Laboratory of Polymer Materials, College of Chemistry and Materials Science, Fuzhou 350007, China.
Nanomaterials (Basel). 2018 Aug 6;8(8):596. doi: 10.3390/nano8080596.
Heavy metal pollution has long been the focus of attention because of its serious threat to human health and the environment. Surface enhanced Raman spectroscopy (SERS) has shown great potential for metal detection owing to many advantages, including, requiring fewer samples, its minimal damage to specimen, and its high sensitivity. In this work, we proposed a simple and distinctive method, based on SERS, using facile silver film (Ag-film) combined with a DNA molecular switch, which allowed for the highly specific detection of heavy metal mercury ions (Hg). When in the presence of Hg ions, the signals from Raman probes attach to single-stranded DNA, which will be dramatically enhanced due to the specific structural change of DNA strands-resulting from the interaction between Hg ions and DNA bases. This SERS sensor could achieve an ultralow limit of detection (1.35 × 10 M) for Hg detection. In addition, we applied this SERS sensor to detect Hg in real blood samples. The results suggested that this SERS platform could be a promising alternative tool for Hg detection in clinical, environmental, and food inspection.
长期以来，重金属污染因其对人类健康和环境的严重威胁而备受关注。表面增强拉曼光谱（SERS）由于具有许多优点，如所需样品较少、对样品损伤最小以及灵敏度高，在金属检测方面显示出巨大潜力。在这项工作中，我们基于SERS提出了一种简单且独特的方法，使用简便的银膜（Ag膜）与DNA分子开关相结合，实现了对重金属汞离子（Hg）的高特异性检测。当存在Hg离子时，附着在单链DNA上的拉曼探针信号会因Hg离子与DNA碱基之间的相互作用导致DNA链的特定结构变化而显著增强。这种SERS传感器对Hg检测可实现超低检测限（1.35×10⁻¹⁰ M）。此外，我们将这种SERS传感器应用于实际血液样本中Hg的检测。结果表明，这个SERS平台可能成为临床、环境和食品检测中Hg检测的一种有前景的替代工具。
