College of Veterinary Medicine, Western University of Health Sciences, Pomona, California.
Graduate College of Biomedical Sciences, Western University of Health Sciences, Pomona, California.
Am J Trop Med Hyg. 2018 Oct;99(4):840-843. doi: 10.4269/ajtmh.17-0740.
is an emerging zoonotic pathogen present in the United States, South America, and Europe. The molecular detection of frequently relies on polymerase chain reaction (PCR) assays that target the genus coupled with DNA sequencing for species determination. However, the presence of other spp. in the sample being tested may result in false-negative results for , especially when Sanger sequencing is used. We developed a sensitive and specific quantitative PCR platform for by targeting the intergenic transcribed spacer, , and genes, which are recommended for subtyping characterization. This PCR platform achieved the limit of detection between five and 10 genomic equivalents per reaction and did not amplify DNA from other species or selected hosts. This PCR platform is a fast and cost-effective option to be used in epidemiological evaluations of reservoirs and vectors and in detecting and quantifying infection in humans.
是一种新兴的人畜共患病病原体，存在于美国、南美洲和欧洲。该病原体的分子检测通常依赖于聚合酶链反应（PCR）检测，该检测靶向属 ，并结合 DNA 测序进行物种鉴定。然而，在被检测的样本中其他 的存在可能导致 的假阴性结果，特别是当使用 Sanger 测序时。我们通过靶向种间转录间隔区、 和 基因，开发了一种针对 的敏感和特异的定量 PCR 平台，该平台建议用于亚型特征描述。该 PCR 平台在每个反应中实现了 5 到 10 个基因组当量的检测限，并且不扩增来自其他 物种或选定宿主的 DNA。该 PCR 平台是一种快速且具有成本效益的选择，可用于对储存宿主和传播媒介进行流行病学评估，以及检测和定量人类感染 。
