Mizuno Y, Itohara S
Am J Vet Res. 1986 Mar;47(3):551-6.
An enzyme-linked immunosorbent assay (ELISA) was developed to detect antibodies against avian leukosis viruses (ALV), using antigens extracted from Rous-associated virus-inoculated chicken embryo fibroblast (CEF) cells by Nonidet P-40 treatments. The antigens reacted strongly to sera of chickens immunized with antigenically homologous viruses, but weakly to those of chickens immunized with heterologous viruses. Antigens extracted from noninoculated CEF cells by the same procedures did not react to either of the immune sera. Normal control sera did not react to any of the antigens. Reactivities of immune sera were decreased markedly by the sera adsorbing with homologous Rous-associated virus-inoculated CEF cells, but not with heterologous CEF cells. The ELISA-specific optimal doses (the differences between the optimal doses with antigens from ALV-inoculated and noninoculated CEF cells) were correlated strongly with the virus-neutralization titers (r = 0.876, P less than 0.01). Examination of the antibody response from ALV-inoculated chickens revealed that ELISA detected antibodies at the same time or several weeks earlier than did the virus-neutralization test.
开发了一种酶联免疫吸附测定（ELISA）来检测抗禽白血病病毒（ALV）抗体，使用通过Nonidet P - 40处理从接种劳斯相关病毒的鸡胚成纤维细胞（CEF）中提取的抗原。这些抗原与用抗原同源病毒免疫的鸡的血清强烈反应，但与用异源病毒免疫的鸡的血清反应较弱。通过相同程序从未接种的CEF细胞中提取的抗原与任何一种免疫血清均无反应。正常对照血清与任何抗原均无反应。免疫血清与接种同源劳斯相关病毒的CEF细胞吸附后反应性明显降低，但与异源CEF细胞吸附后则无此现象。ELISA特异性最佳剂量（接种ALV的CEF细胞抗原与未接种的CEF细胞抗原的最佳剂量之差）与病毒中和滴度密切相关（r = 0.876，P小于0.01）。对接种ALV的鸡的抗体反应进行检测发现，ELISA检测到抗体的时间与病毒中和试验相同或早几周。
