Centre for Marine Bioproducts Development, College of Medicine and Public Health, Flinders University, Bedford Park, SA, 5042, Adelaide, Australia.
Medical Biotechnology, College of Medicine and Public Health, Flinders University, Bedford Park, SA, 5042, Adelaide, Australia.
Sci Rep. 2018 Aug 7;8(1):11801. doi: 10.1038/s41598-018-30295-y.
Sponge-bacteria interactions are very important due to their ecological and biological significance. To understand the impact of interactions between sponges and bacteria (both associated with and external to sponges) on sponge-associated microbial diversity, sponge metabolite profiles and bioactivity, we used a controlled aquarium system and designed an experimental approach that allows the study of sponge-bacteria interactions in a well-defined manner. To test the feasibility of this approach, this system was used to study the interaction between a sponge Aplysilla rosea and a marine bacterium commonly found in seawater, Vibrio natriegens. Sponge explants were exposed to V. natriegens, at 5 × 10 cfu/ml, and changes were monitored for 48 hours. Pyro-sequencing revealed significant shifts in microbial communities associated with the sponges after 24 to 48 hours. Both the control (sponge only without added bacteria) and Vibrio-exposed sponges showed a distinct shift in bacterial diversity and abundance with time. Vibrio exposure significantly increased bacterial diversity, the abundance of a number of taxa compared to control sponges. The result experimentally supports the notion of dynamic and concerted responses by the sponge when interacting with a bacterium, and demonstrates the feasibility of using this controlled aquarium system for the study of sponge-bacteria interactions.
海绵-细菌相互作用非常重要，因为它们具有生态和生物学意义。为了了解海绵与细菌（包括海绵内和海绵外的细菌）之间的相互作用对海绵相关微生物多样性、海绵代谢物谱和生物活性的影响，我们使用了一个受控水族系统，并设计了一种实验方法，可以以明确定义的方式研究海绵-细菌相互作用。为了测试这种方法的可行性，该系统用于研究海绵 Aplysilla rosea 与常见于海水中的海洋细菌 Vibrio natriegens 之间的相互作用。将海绵外植体暴露于 5×10 cfu/ml 的 Vibrio natriegens 中，并监测 48 小时的变化。焦磷酸测序显示，海绵在 24 至 48 小时后与海绵相关的微生物群落发生了显著变化。对照组（仅海绵，无添加细菌）和 Vibrio 暴露的海绵随时间推移显示出细菌多样性和丰度的明显变化。与对照组海绵相比，Vibrio 暴露显著增加了细菌多样性和一些分类群的丰度。实验结果从实际上支持了海绵与细菌相互作用时动态和协同反应的观点，并证明了使用这种受控水族系统研究海绵-细菌相互作用的可行性。
