Takimoto T, Sato H, Ogura H, Miyawaki T, Glaser R
Cancer Res. 1986 May;46(5):2541-4.
Three Epstein-Barr virus (EBV) genome-positive epithelial/hybrid cell lines (D98/HR-1, NPC-KT, and A2L/AH) were superinfected with EBV derived from P3HR-1 (HR-1), NPC-KT, and B95-8 cells. The HR-1 virus is lytic and induces early antigen in superinfected Raji cells; the virus is not capable of transforming B-lymphocytes. Virus preparations from NPC-KT cells have both transforming and early antigen-inducing properties, while B95-8 virus can only transform B-lymphocytes. It was possible to demonstrate EBV antigens after superinfection of D98/HR-1 cells with both HR-1 virus and NPC-EBV. The NPC-KT hybrid cells, which were originally prepared by fusing human adenoid epithelial cells (Ad-AH) and EBV genome-positive NPC explanted epithelial cells, were susceptible to superinfection with HR-1 virus but not to NPC-EBV. The A2L/AH hybrid cells, which were prepared by fusion between Ad-AH cells and lymphocytes transformed by B95-8 virus, could not be superinfected with any of the virus preparations. In order to further investigate the nature of the EBV receptor as it relates to other cell membrane components, we examined cell surface markers on Ad-AH, NPC-KT, A2L/AH, and D98/HR-1 cells using monoclonal antibodies and by rosette formation. We found that the NPC-KT, A2L/AH, and Ad-AH cell lines express the OKB2 antigen and that the common acute lymphoblastic leukemia antigen is expressed on the A2L/AH cells. We also found that NPC-KT parental cells and a clone of NPC-KT cells express erythrocyte antibody complement b and erythrocyte antibody complement d, as determined by rosette formation, but were negative for C3b and C3d when monoclonal antibodies against these two markers were used. The D98/HR-1 cells were also confirmed to be negative for C3b and C3d. The data suggest that the C3d receptor may be part of the EBV receptor but that the C3d receptor, by itself, is not the only receptor to which EBV can bind.
三种爱泼斯坦-巴尔病毒（EBV）基因组阳性的上皮/杂交细胞系（D98/HR-1、NPC-KT和A2L/AH）分别被源自P3HR-1（HR-1）、NPC-KT和B95-8细胞的EBV超感染。HR-1病毒具有裂解性，可在超感染的拉吉细胞中诱导早期抗原；该病毒无转化B淋巴细胞的能力。来自NPC-KT细胞的病毒制剂兼具转化和诱导早期抗原的特性，而B95-8病毒只能转化B淋巴细胞。用HR-1病毒和NPC-EBV对D98/HR-1细胞进行超感染后，均能检测到EBV抗原。NPC-KT杂交细胞最初是通过将人腺样体上皮细胞（Ad-AH）与EBV基因组阳性的鼻咽癌外植上皮细胞融合制备的，它易被HR-1病毒超感染，但不易被NPC-EBV超感染。A2L/AH杂交细胞是通过Ad-AH细胞与被B95-8病毒转化的淋巴细胞融合制备的，不能被任何一种病毒制剂超感染。为了进一步研究EBV受体与其他细胞膜成分相关的性质，我们使用单克隆抗体并通过玫瑰花结形成检测了Ad-AH、NPC-KT、A2L/AH和D98/HR-1细胞的细胞表面标志物。我们发现NPC-KT、A2L/AH和Ad-AH细胞系表达OKB2抗原，并且A2L/AH细胞表达常见的急性淋巴细胞白血病抗原。我们还发现，通过玫瑰花结形成测定，NPC-KT亲代细胞和一个NPC-KT细胞克隆表达红细胞抗体补体b和红细胞抗体补体d，但当使用针对这两种标志物的单克隆抗体时，C3b和C3d呈阴性。D98/HR-1细胞也被证实C3b和C3d呈阴性。数据表明C3d受体可能是EBV受体的一部分，但C3d受体本身并非EBV能够结合的唯一受体。
