Randhawa P S, Jaffe R, Demetris A J, Nalesnik M, Starzl T E, Chen Y Y, Weiss L M
Department of Pathology, Presbyterian University Hospital, Pittsburgh, Pennsylvania 15213.
Am J Pathol. 1991 Apr;138(4):1027-33.
The systemic distribution of Epstein-Barr virus (EBV) genomes was studied in paraffin-embedded tissues from 12 fatal cases of Post-transplantation lymphoproliferative disease (PTLD), using an in situ hybridization technique employing an alpha-35S-dCTP-radiolabeled BamHI-W fragment of EBV DNA. The presence of EBV was documented in various PTLD-involved organs. The hybridization signal for the virus localized predominantly in the abnormal lymphoid cells, but signals also were detected in hepatocytes and/or adrenal cortical cells in five cases. The distribution of autoradiographic label within the lymphoid cells was focal and its intensity varied from field to field suggesting a nonuniformity of the viral genomic load in the infected tissues. Recruitment of EBV genome-bearing cells was not observed into inflammatory mononuclear infiltrates found in organs without histopathologic evidence of PTLD.
利用原位杂交技术，采用EBV DNA的α-35S-dCTP放射性标记的BamHI-W片段，对12例移植后淋巴细胞增生性疾病（PTLD）死亡病例的石蜡包埋组织中EBV基因组的全身分布进行了研究。在各种PTLD累及的器官中均证实了EBV的存在。病毒的杂交信号主要定位于异常淋巴细胞，但在5例患者的肝细胞和/或肾上腺皮质细胞中也检测到了信号。放射自显影标记在淋巴细胞内的分布是局灶性的，其强度因视野而异，提示受感染组织中病毒基因组负荷不均匀。在没有PTLD组织病理学证据的器官中发现的炎性单核细胞浸润中，未观察到携带EBV基因组的细胞的募集。
