Chester J F, Gaissert H A, Ross J S, Malt R A
Cancer Res. 1986 Jun;46(6):2954-7.
Because epidermal growth factor (EGF) is rapidly bound and internalized into rat pancreas, stimulates uptake of tritiated thymidine, and increases pancreatic weight, a cocarcinogenic effect on pancreatic cancer seemed likely. Pancreatic adenocarcinomas were induced in 70 female Syrian hamsters by 19 weekly s.c. injections of N-nitrosobis(2-oxopropyl)amine (BOP) (10 mg/kg). From Wk 5 through Wk 8 of BOP injections, additional s.c. injections of EGF (5 micrograms every 3 days for 10 injections) were given to 45 animals, while 25 received saline solution. An additional group of 10 received EGF alone, and another 10 animals received saline solution alone (controls). Eleven wk later, the mean body weight of EGF-treated animals increased by 29% as compared with that of controls, and their mean pancreatic weight relative to body weight increased by 44% as compared with controls. The mean body weight of EGF + BOP-treated animals increased by 10%, and their pancreatic weight relative to body weight increased by 22% as compared with that of animals treated with BOP alone. The incidence of pancreatic cancer in the EGF + BOP-treated animals was 75% versus 44% in those treated with BOP alone (P = 0.016). No tumors developed in either animals treated with EGF alone or control animals. EGF augments pancreatic carcinogenesis induced by BOP. The incidence of bronchial carcinomas doubles.
由于表皮生长因子（EGF）能迅速与大鼠胰腺结合并被内化，刺激氚标记胸腺嘧啶核苷的摄取，并增加胰腺重量，因此它似乎有可能对胰腺癌产生促癌作用。通过每周1次、连续19次皮下注射N-亚硝基双（2-氧代丙基）胺（BOP，10毫克/千克），在70只雌性叙利亚仓鼠中诱发胰腺腺癌。在BOP注射的第5周和第8周期间，对45只动物额外进行皮下注射EGF（每3天5微克，共注射10次），而25只动物注射生理盐水。另外一组10只动物仅接受EGF注射，另有10只动物仅接受生理盐水注射（作为对照）。11周后，与对照组相比，接受EGF治疗的动物平均体重增加了29%，其相对于体重的平均胰腺重量增加了44%。与仅接受BOP治疗的动物相比，接受EGF + BOP治疗的动物平均体重增加了10%，其相对于体重的胰腺重量增加了22%。接受EGF + BOP治疗的动物胰腺癌发病率为75%，而仅接受BOP治疗的动物为44%（P = 0.016）。仅接受EGF治疗的动物和对照动物均未发生肿瘤。EGF增强了由BOP诱导的胰腺癌发生。支气管癌的发病率增加了一倍。
