Höper Tessa, Mussotter Franz, Haase Andrea, Luch Andreas, Tralau Tewes
German Federal Institute for Risk Assessment , Department of Chemical and Product Safety , Max-Dohrn-Strasse 8-10 , 10589 Berlin , Germany . Email:
Toxicol Res (Camb). 2017 Jun 13;6(5):595-610. doi: 10.1039/c7tx00058h. eCollection 2017 Sep 1.
Allergic contact dermatitis (ACD) is a widespread hypersensitivity reaction of the skin. The cellular mechanisms underlying its development are complex and involve close interaction of different cell types of the immune system. It is this very complexity which has long prevented straightforward replacement of the corresponding regulatory tests. Recent efforts have already resulted in the development of several testing alternatives that address key steps of ACD. Yet identification of suitable biomarkers is still a subject of intense research. Search strategies for the latter encompass transcriptomics, proteomics as well as metabolomics approaches. The scope of this review shall be the application and use of proteomics in the context of ACD. This includes highlighting relevant aspects of the molecular and cellular mechanisms underlying ACD, the exploitation of these mechanisms for testing and biomarkers (, in the context of the OECD's adverse outcome pathway initiative) as well as an outlook on emerging proteome targets, for example during the allergen-induced activation of dendritic cells (DCs).
过敏性接触性皮炎（ACD）是一种广泛存在的皮肤超敏反应。其发生的细胞机制复杂，涉及免疫系统不同细胞类型之间的密切相互作用。正是这种复杂性长期以来阻碍了相应常规检测方法的直接替代。最近的努力已经促成了几种针对ACD关键步骤的检测替代方法的开发。然而，合适生物标志物的鉴定仍然是深入研究的课题。寻找生物标志物的策略包括转录组学、蛋白质组学以及代谢组学方法。本综述的范围将是蛋白质组学在ACD背景下的应用。这包括突出ACD潜在的分子和细胞机制的相关方面，在经合组织不良结局途径倡议背景下利用这些机制进行检测和寻找生物标志物，以及对新兴蛋白质组靶点（例如在变应原诱导的树突状细胞（DC）激活过程中）的展望。
