Liquid-chromatographic determination of isoenzymes of alkaline phosphatase in serum and tissue homogenates.
作者信息
Schoenau E, Herzog K H, Boehles H J
出版信息
Clin Chem. 1986 May;32(5):816-8.
PMID:3009053
Abstract
We describe a new method for separating alkaline phosphatase (AP) isoenzymes by means of "high-performance" liquid chromatography. Isoenzymes are eluted from the column (Mono Q HR 5/5, a strong anion-exchanger) with a stepwise gradient of LiCl. The isoenzymes originating from small intestine, bone, liver, and bile were identified by use of tissue homogenates, pathological sera, and heat inactivation.
摘要
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