高分子量碱性磷酸酶：通过液相色谱法进行简化且高灵敏度的测定

High-molecular-mass alkaline phosphatase: simplified and highly sensitive determination by liquid chromatography.

作者信息

Schoenau E, Boeswald W, Herzog K H, Scheele J, Boehles H J, Stehr K

机构信息

Klinik mit Poliklinik für Kinder und Jugendliche der Friedrich-Alexander Universität, Erlangen, F.R.G.

出版信息

Clin Chem. 1988 Oct;34(10):2130-3.

PMID:3168231

Abstract

This simplified HPLC method for measurement of high-molecular-mass alkaline phosphatase (high-Mr AP; EC 3.1.3.1) in serum and bile is rapid (time for column preparation and separation 30 min), reproducible (CV 4.2%), and highly sensitive (detects high-Mr AP in healthy controls at 1-3% of total AP activity in serum), and is suitable for processing small batches of sample. We characterized high-Mr AP in serum and bile by incubating samples with L-phenylalanine, neuraminidase, 1-butanol, or wheat-germ lectin, and by determining stability to heat. High-Mr AP activity was determined in sera of patients with various liver diseases (4-32% of total AP serum activity) and results were compared with those by electrophoresis on agarose.

摘要

这种用于测定血清和胆汁中高分子量碱性磷酸酶（高Mr AP；EC 3.1.3.1）的简化高效液相色谱法快速（柱制备和分离时间为30分钟）、可重复（CV 4.2%）且高度灵敏（能检测出健康对照者血清中高Mr AP占总AP活性的1 - 3%），适用于处理小批量样本。我们通过用L - 苯丙氨酸、神经氨酸酶、1 - 丁醇或麦胚凝集素孵育样本并测定热稳定性来对血清和胆汁中的高Mr AP进行表征。测定了各种肝病患者血清中的高Mr AP活性（占血清总AP活性的4 - 32%），并将结果与琼脂糖电泳结果进行比较。