Gudkov A V, Korec E, Chernov M V, Tikhonenko A T, Obukh I B, Hlozánek I
Folia Biol (Praha). 1986;32(1):65-72.
Seven loci of endogenous proviruses were detected in the genome of Brown Leghorn chickens. Sets of endogenous proviruses in DNA of the chicken embryos examined were identified by blot hybridization with 32P-labelled DNA of RSV and EcoRI restriction endonuclease digestion. Comparison of the results showed that only one locus (A) of endogeneous provirus was associated with a gs+ phenotype as determined by the immunoperoxidase reaction and antibodies against gag gene products of RSV. Restriction endonuclease analysis with HindIII, BamHI and SacI revealed that proviruses A and F in Brown Leghorn chickens correspond to loci ev-3 and ev-6, respectively, in White Leghorn chickens. Other loci (B, C, D, E, and X) were designated ev-22, ev-23, ev-24, ev-25, ev-26, respectively. None of these loci expressed infectious virions. The structure of most of the endogenous proviruses examined is considerably different from the genome of the endogenous chicken virus RAV-O. The difference in structure may be one possible cause of the absence of endogenous provirus expression.
在白来航鸡的基因组中检测到七个内源性前病毒位点。通过用32P标记的劳氏肉瘤病毒（RSV）DNA进行斑点杂交和EcoRI限制性内切酶消化，鉴定了所检测鸡胚DNA中的内源性前病毒组。结果比较表明，通过免疫过氧化物酶反应和针对RSV gag基因产物的抗体测定，只有一个内源性前病毒位点（A）与gs+表型相关。用HindIII、BamHI和SacI进行的限制性内切酶分析表明，白来航鸡中的前病毒A和F分别对应于白来航鸡中的ev-3和ev-6位点。其他位点（B、C、D、E和X）分别命名为ev-22、ev-23、ev-24、ev-25、ev-26。这些位点均未表达传染性病毒粒子。所检测的大多数内源性前病毒的结构与内源性鸡病毒RAV-O的基因组有很大不同。结构差异可能是内源性前病毒不表达的一个可能原因。
