Gudkov A V, Obukh I B, Serov S M, Naroditsky B S
J Gen Virol. 1981 Nov;57(Pt 1):85-94. doi: 10.1099/0022-1317-57-1-85.
We have compared endogenous proviruses in DNA of chickens of 11 breeds by means of Southern's technique. Many of the endogenous virus loci found were missing from the genomes of the extensively studied white leghorn chickens, although some of the proviruses and especially ev-1 appeared to be widely spread among different chickens. Most of the proviruses were similar to Rous-associated virus (RAV-O) as judged by the EcoRI digestion patterns. Several genetically different proviruses were found in the DNA of 28 brown leghorns. They contain neither ev-1 provirus in their genome, nor any other one common for all individuals. All four Italian partridge-coloured chickens examined appeared to be free from ALV-related sequences in their DNA showing the possibility of normal life without known endogenous proviruses. Possible causes of inter-breed differences of chicken endogenous proviruses are discussed.
我们运用Southern技术比较了11个品种鸡的DNA中的内源性前病毒。尽管部分前病毒，尤其是ev-1，似乎在不同鸡种中广泛传播，但在广泛研究的白来航鸡基因组中缺失了许多所发现的内源性病毒位点。根据EcoRI酶切图谱判断，大多数前病毒与劳氏相关病毒（RAV-O）相似。在28只褐来航鸡的DNA中发现了几种基因不同的前病毒。它们的基因组中既不包含ev-1前病毒，也没有所有个体共有的任何其他前病毒。所检测的所有四只意大利鹧鸪色鸡的DNA中似乎都没有与禽白血病病毒（ALV）相关的序列，这表明在没有已知内源性前病毒的情况下也有可能正常生活。文中讨论了鸡内源性前病毒品种间差异的可能原因。
