College of Food Science and Pharmacology, Xinjiang Agricultural University, Xinjiang, 830052, China; Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection/Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Huaiyin Normal University, Huaian, 223300, China.
Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection/Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Huaiyin Normal University, Huaian, 223300, China.
Carbohydr Polym. 2018 Oct 15;198:537-545. doi: 10.1016/j.carbpol.2018.06.121. Epub 2018 Jun 30.
Ginsenoside compound K (CK) has been shown to exhibit anticancer properties. In this study, chitosan nanoparticles loaded with ginsenoside compound K (CK-NPs) were prepared as a delivery system using a self-assembly technique with amphipathic deoxycholic acid-O carboxymethyl chitosan as the carrier, which improved the water solubility of CK. By evaluating drug loading, entrapment efficiency, and in vitro release behavior, the feasibility of CK-NPs as a drug carrier nanoparticle for the treatment of human hepatic carcinoma cells (HepG2) was investigated. Result revealed that CK and CK-NPs showed a dose-dependent inhibitory effect on HepG2 cells with IC values of 23.33 and 16.58 μg/mL, respectively. Furthermore, fluorescence imaging demonstrated that CK-NPs promoted cellular uptake in vitro. Therefore, all results indicated that CK-NPs might be a novel drug delivery system to improve the solubility and enhance the cytotoxic and apoptotic potentials of CK for effective liver cancer chemotherapy.
人参皂苷化合物 K（CK）已被证明具有抗癌特性。在这项研究中，采用自组装技术，以两亲性去氧胆酸-O-羧甲基壳聚糖为载体，制备了负载人参皂苷化合物 K（CK-NPs）的壳聚糖纳米粒子作为递送系统，提高了 CK 的水溶性。通过评估载药量、包封率和体外释放行为，研究了 CK-NPs 作为治疗人肝癌细胞（HepG2）的药物载体纳米粒子的可行性。结果表明，CK 和 CK-NPs 对 HepG2 细胞表现出剂量依赖性抑制作用，IC 值分别为 23.33 和 16.58μg/mL。此外，荧光成像显示 CK-NPs 促进了细胞摄取。因此，所有结果表明 CK-NPs 可能是一种新的药物递送系统，可提高 CK 的溶解度，并增强其细胞毒性和促凋亡潜力，从而有效进行肝癌化疗。
