Department of Analytical Chemistry, Faculty of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Analytical Chemistry, Faculty of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
J Chromatogr A. 2018 Oct 12;1571:38-46. doi: 10.1016/j.chroma.2018.08.005. Epub 2018 Aug 2.
This study introduced a developed approach for dendritic β-cyclodextrin (β-CD) in order to obtain high sorption capacity. Synthetic strategy exploits the reactivity between acrylic acid and allyl glycidyl ether for high-yielding assembly via grafting on to the magnetic nanoparticles that are modified using 3-mercaptopropyltrimethoxysilane for various building branches and host-guest molecules of β-CD. The methodology has been applied for the preparation of a series of β-CD conjugated magnetic nanoparticles with dendrimers as a nano-sorbent for the extraction of methylprednisolone acetate. This study allowed us to probe (i) the effects of the dendric-cyclodextrin architecture on the affinity of sorption capacity, (ii) the drug influence between the cyclodextrin core and the polyester dendrimer, and (iii) the result of sorbent formation for using the anti-inflammatory drug as a target guest into the ring of β-CD on biological extraction. It was found that the adsorption behavior could be fitted by the Langmuir adsorption isotherm model. The adsorption capacity of MPA is found to be 12.4 mg g and indicated the homogeneous sites onto polymer grafted magnetite nano-sorbent surface. Our results confirm the high capability of this type of dendrimer-β-CD for drug extraction in biological fluids and pharmaceutical samples. This nano-sorbent assists the magnetic solid phase extraction technique represented in the high extraction yield (up to 97%) for methylprednisolone acetate in biological human fluids and pharmaceutical samples. Moreover, the achieved polymeric nano-sorbent of the reaction combination was facilitated by a magnetic field and reusability was performed without any notable loss in the sorbent activity.
本研究介绍了一种开发的树枝状β-环糊精（β-CD）方法，以获得高吸附能力。合成策略利用丙烯酸与烯丙基缩水甘油醚之间的反应性，通过接枝到经过 3-巯丙基三甲氧基硅烷修饰的磁性纳米粒子上，实现高产率的组装，以获得各种支链和β-CD 的主体客体分子。该方法已应用于一系列β-CD 接枝磁性纳米粒子的制备，作为纳米吸附剂，用于提取醋酸甲泼尼龙。本研究使我们能够探测：（i）树枝状-环糊精结构对吸附容量亲和力的影响；（ii）环糊精核与聚酯树枝状聚合物之间的药物影响；（iii）作为目标客体的抗炎药物进入β-CD 环的吸附剂形成对生物提取的影响。结果表明，吸附行为可以通过朗缪尔吸附等温线模型拟合。发现 MPA 的吸附容量为 12.4mg g，并表明聚合物接枝磁铁矿纳米吸附剂表面上存在均匀的吸附位点。我们的结果证实了这种类型的树枝状聚合物-β-CD 对生物流体和药物样品中药物提取的高能力。这种纳米吸附剂辅助了磁性固相萃取技术，在生物人液和药物样品中，对醋酸甲泼尼龙的萃取收率高达 97%。此外，通过磁场促进了反应组合的聚合物纳米吸附剂的形成，并可重复使用，而吸附剂活性没有明显损失。
