Department of Chemistry, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Talanta. 2013 Dec 15;117:511-7. doi: 10.1016/j.talanta.2013.09.015. Epub 2013 Oct 3.
In this paper, a new method is reported for the surface grafting of poly[1-(N,N-bis-carboxymethyl)amino-3-allylglycerol-co-dimethylacrylamide] onto magnetic nano-particles modified by 3-mercaptopropyltrimethoxysilane. The grafted nano-sorbent was characterized by Fourier transform infrared spectroscopy, elemental analysis, thermogravimetric analysis, and scanning electron microscopy. Agglomerated nano-particles with multi-pores were used for extraction and determination of trace letrozole in human biological fluids and pharmaceutical samples. The profile of the letrozole uptake by the magnetic nano-sorbent reflected good accessibility of the active sites in the grafted polymer. Scatchard analysis revealed that the sorption capacity of the functionalized nano-sorbent was 6.27 µmol g(-1) at an optimum pH of 4. The equilibrium adsorption data of letrozole by grafted magnetic nano-sorbent were analyzed by Langmuir, Freundlich, Temkin and Redlich-Peterson models. Conformation of the experimental data in the Langmuir isotherm model indicated the homogeneous binding site of functional polymer-grafted magnetic nano-sorbent surface. Nearly 89% of letrozole was released in simulated gastric fluid, pH 1.2, in 2h and 79% in simulated intestinal fluid, pH 7.4, in 30 h. These results show the utility of the letrozole loaded- polymer grafted magnetite nano-particles for enteric drug delivery.
本文报道了一种新的方法，用于通过 3-巯丙基三甲氧基硅烷改性的磁性纳米粒子表面接枝聚[1-（N，N-双羧甲基）氨基-3-烯丙基甘油-co-二甲基丙烯酰胺]。接枝纳米吸附剂通过傅里叶变换红外光谱、元素分析、热重分析和扫描电子显微镜进行了表征。多微孔的团聚纳米粒子用于提取和测定人生物流体和药物样品中的痕量来曲唑。磁性纳米吸附剂对来曲唑的摄取曲线反映了接枝聚合物中活性位点的良好可及性。Scatchard 分析表明，在最佳 pH 值为 4 时，功能化纳米吸附剂的吸附容量为 6.27 µmol g(-1)。接枝磁性纳米吸附剂对来曲唑的平衡吸附数据通过 Langmuir、Freundlich、Temkin 和 Redlich-Peterson 模型进行了分析。实验数据符合 Langmuir 等温模型的构象表明，功能聚合物接枝磁性纳米吸附剂表面具有均匀的结合位点。在模拟胃液 pH 1.2 下，约 89%的来曲唑在 2h 内释放，在模拟肠液 pH 7.4 下，在 30h 内释放 79%。这些结果表明，载来曲唑的聚合物接枝磁铁矿纳米粒子可用于肠内药物传递。
