Mesa-Pereira Beatriz, Rea Mary C, Cotter Paul D, Hill Colin, Ross R Paul
Teagasc Food Research Centre, Teagasc Moorepark, Fermoy, Cork, Ireland.
APC Microbiome Ireland, University College Cork, Cork, Ireland.
Front Microbiol. 2018 Jul 26;9:1654. doi: 10.3389/fmicb.2018.01654. eCollection 2018.
Bacteriocins, a heterogenous group of antibacterial ribosomally synthesized peptides, have potential as bio-preservatives in in a wide range of foods and as future therapeutics for the inhibition of antibiotic-resistant bacteria. While many bacteriocins have been characterized, several factors limit their production in large quantities, a requirement to make them commercially viable for food or pharma applications. The identification of new bacteriocins by database mining has been promising, but their potential is difficult to evaluate in the absence of suitable expression systems. has been used as a heterologous host to produce recombinant proteins for decades and has an extensive set of expression vectors and strains available. Here, we review the different expression systems for bacteriocin production using this host and identify the most important features to guarantee successful production of a range of bacteriocins.
细菌素是一类由核糖体合成的抗菌肽,种类多样,在广泛的食品中具有作为生物防腐剂的潜力,并且有望成为未来抑制抗生素抗性细菌的治疗剂。虽然许多细菌素已被鉴定,但有几个因素限制了它们的大量生产,而大量生产是使其在食品或制药应用中具有商业可行性的必要条件。通过数据库挖掘鉴定新的细菌素很有前景,但在缺乏合适表达系统的情况下,其潜力难以评估。几十年来,(此处原文缺失具体宿主名称)已被用作异源宿主来生产重组蛋白,并且有大量可用的表达载体和菌株。在这里,我们综述了使用该宿主生产细菌素的不同表达系统,并确定了确保成功生产一系列细菌素的最重要特征。