Klein Claudia, Barua Shemanti, Liccioli Stefano, Massolo Alessandro
1 Department of Veterinary Clinical and Diagnostic Sciences, Faculty of Veterinary Medicine, University of Calgary, 3280 Hospital Drive NW, Calgary, Alberta AB T2N 4Z6, Canada.
2 Department of Biological Sciences, Faculty of Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alberta AB T2N 1N4, Canada.
J Wildl Dis. 2019 Jan;55(1):196-199. doi: 10.7589/2018-02-027. Epub 2018 Aug 10.
Definitive hosts of Neospora caninum are species of canids, such as domestic dogs ( Canis lupus familiaris), coyotes ( Canis latrans) and foxes ( Vulpes vulpes), whereas ruminants, such as cattle ( Bos taurus), sheep ( Ovis aries), and deer (Cervidae) serve as intermediate hosts. We investigated the presence of N. caninum in feces of coyotes in Calgary, Alberta, Canada. One hundred coyote fecal samples were collected from five city parks. Following DNA extraction, the presence of N. caninum DNA was determined by using real-time PCR analysis with a primer and probe pair targeting the Nc5 gene. Ten of the 100 samples contained detectable amounts of N. caninum DNA. White-tailed deer ( Odocoileus virginianus) and mule deer ( Odocoileus hemionus) are common in Calgary's city parks, and although we did not examine them for the presence of N. caninum bradyzoites, the presence of N. caninum DNA in coyote fecal samples is indicative that a sylvatic host of N. caninum exists within this urban environment.
犬新孢子虫的终末宿主是犬科动物，如家养犬（犬狼疮家犬）、郊狼（犬属拉氏）和狐狸（赤狐），而反刍动物，如牛（牛属）、绵羊（绵羊属）和鹿（鹿科）则作为中间宿主。我们调查了加拿大艾伯塔省卡尔加里市郊狼粪便中犬新孢子虫的存在情况。从五个城市公园收集了100份郊狼粪便样本。DNA提取后，使用针对Nc5基因的引物和探针组通过实时PCR分析确定犬新孢子虫DNA的存在。100个样本中有10个含有可检测量的犬新孢子虫DNA。白尾鹿（弗吉尼亚鹿）和骡鹿（黑尾鹿）在卡尔加里市公园很常见，虽然我们没有检查它们是否存在犬新孢子虫缓殖子，但郊狼粪便样本中犬新孢子虫DNA的存在表明在这个城市环境中存在犬新孢子虫的野生动物宿主。
