Bandelj Petra, Kušar Darja, Šimenc Laura, Jamnikar-Ciglenečki Urška, Vengušt Gorazd, Vengušt Diana Žele
Institute of Microbiology and Parasitology, Veterinary Faculty, University of Ljubljana, Gerbičeva ulica 60, 1115 Ljubljana, Slovenia.
Institute of Food Safety, Feed and Environment, Veterinary Faculty, University of Ljubljana, Gerbičeva ulica 60, 1115 Ljubljana, Slovenia.
Animals (Basel). 2023 Oct 3;13(19):3089. doi: 10.3390/ani13193089.
is an obligate intracellular parasite that causes reproductive disorders and major economic losses in cattle, and induces neuromuscular disorders in canids. Exogenous infections are becoming increasingly important due to disease outbreaks. The sylvatic life cycle of interferes with the domestic dog-ruminant life cycle, but understanding of it is scarce. The population of wild canids may play an important role in parasite dispersion. Feces from 42 grey wolves () and 39 golden jackals () were analyzed for the Nc5 gene using a novel real-time PCR (qPCR) with a detection limit of 5 targets/µL in clinical samples. Three wolves (3/42; 7.1%) and one golden jackal (1/39; 2.6%) tested positive, which is the first detection of in the population of grey wolves in Slovenia and the first detection of DNA in the feces of a golden jackal. In addition to the grey wolf, we propose the golden jackal as a potential definitive host with hypothetical epidemiological importance for the sylvatic-domestic life cycle of , due to its proximity to human habitats and its rapid expansion throughout Europe.
是一种专性细胞内寄生虫，可导致牛的生殖障碍和重大经济损失，并引起犬科动物的神经肌肉疾病。由于疾病爆发，外源感染变得越来越重要。的森林生命周期干扰了家犬 - 反刍动物的生命周期，但对其了解甚少。野生犬科动物种群可能在寄生虫传播中发挥重要作用。使用一种新型实时荧光定量聚合酶链反应（qPCR）对42只灰狼（）和39只金豺（）的粪便进行分析，该方法在临床样本中的检测限为每微升5个靶标，以检测Nc5基因。三只狼（3/42；7.1%）和一只金豺（1/39；2.6%）检测呈阳性，这是斯洛文尼亚灰狼种群中首次检测到，也是金豺粪便中首次检测到DNA。除了灰狼，由于金豺靠近人类栖息地且在欧洲迅速扩张，我们提出金豺作为一种潜在的终末宿主，对的森林 - 家养生命周期具有假设的流行病学重要性。
