Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China; Rural Environment Protection Engineering & Technology Center of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China.
Institute of Ecological and Environmental Sciences, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China; Rural Environment Protection Engineering & Technology Center of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China.
Bioresour Technol. 2018 Nov;268:355-362. doi: 10.1016/j.biortech.2018.08.009. Epub 2018 Aug 4.
Phosphoric acid plus hydrogen peroxide (PHP) pretreatment was employed on wheat straw for ethanol conversion by simultaneous saccharification and fermentation (SSF) at high loadings. Results showed solid loading of PHP-pretreated wheat straw can be greatly promoted to 20%. Although more enzyme input improved ethanol conversion significantly, it still can be potentially reduced to 10-20 mg protein/g cellulose. Increasing yeast input also promoted ethanol conversion, however, the responses were not significant. Response surface method was employed to optimize SSF conditions with the strategy of maximizing ethanol conversion and concentration and minimizing enzyme and yeast input. Results indicated that ethanol conversion of 88.2% and concentration of 69.9 g/L were obtained after 120 h SSF at solid loading of 15.3%, and CTec2 enzyme and yeast were in lower input of 13.2 mg protein/g cellulose and 1.0 g/L, respectively. Consequently, 15.5 g ethanol was harvested from 100 g wheat straw in the optimal conditions.
磷酸加过氧化氢（PHP）预处理可在高负荷下通过同步糖化发酵（SSF）将小麦秸秆转化为乙醇。结果表明，PHP 预处理小麦秸秆的固体负荷可大大提高到 20%。尽管更多的酶投入显著提高了乙醇转化率，但仍有可能降低到 10-20mg 蛋白/g 纤维素。增加酵母投入也促进了乙醇转化，但响应不显著。采用响应面法优化 SSF 条件，采用最大化乙醇转化率和浓度、最小化酶和酵母投入的策略。结果表明，在 15.3%的固体负荷下，经过 120h 的 SSF，可获得 88.2%的乙醇转化率和 69.9g/L 的浓度，CTec2 酶和酵母的投入量分别为 13.2mg 蛋白/g 纤维素和 1.0g/L。因此，在最佳条件下，从 100g 小麦秸秆中可收获 15.5g 乙醇。
