Ajayo Pleasure Chisom, Wang Qing, Huang Mei, Zhao Li, Tian Dong, He Jinsong, Fang Dexin, Hu Jinguang, Shen Fei
Institute of Ecological and Environmental Sciences, College of Environmental Sciences, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China; Rural Environment Protection Engineering & Technology Center of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China.
Chemical and Petroleum Engineering, Schulich School of Engineering, The University of Calgary, Calgary T2N 4H9, Canada.
Bioresour Technol. 2023 Apr;374:128759. doi: 10.1016/j.biortech.2023.128759. Epub 2023 Feb 17.
The optimization of key simultaneous saccharification and fermentation (SSF) parameters for bioethanol production from phosphoric acid plus hydrogen peroxide pretreated paper mulberry wood was carried out under two isothermal scenarios; the yeast optimum and trade-off temperatures of 35 and 38 °C, respectively. The optimal conditions established for SSF at 35 °C (solid loading: 16%; enzyme dosage: 9.8 mg protein/g glucan; and yeast concentration: 6.5 g/L) achieved high ethanol titer and yield of 77.34 g/L and 84.60% (0.432 g/g), respectively. These corresponded to 1.2 and 1.3-folds increases, compared to the results of the optimal SSF at a relatively higher temperature of 38 °C. The information from this study would prove beneficial in reducing process energy demands to some extent, while also helping to achieve high levels of both ethanol concentration and yield that are desired in cellulosic ethanol production.
在两种等温条件下，即酵母最佳温度35°C和折衷温度38°C，对磷酸加过氧化氢预处理的构树木材进行生物乙醇生产的关键同步糖化发酵（SSF）参数优化。在35°C下为SSF确定的最佳条件（固体负载量：16%；酶用量：9.8 mg蛋白质/克葡聚糖；酵母浓度：6.5 g/L）分别实现了77.34 g/L的高乙醇滴度和84.60%（0.432 g/g）的乙醇产率。与在相对较高温度38°C下的最佳SSF结果相比，这些分别提高了1.2倍和1.3倍。这项研究的信息将在一定程度上有助于降低工艺能源需求，同时也有助于实现纤维素乙醇生产中所需的高乙醇浓度和产率。
