Department of Pediatric Neurology, Fukuoka Children's Hospital, Fukuoka, Japan; Department of General Pediatrics and Interdisciplinary Medicine, Fukuoka Children's Hospital, Fukuoka, Japan.
Department of Pediatrics, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
J Neurol Sci. 2018 Sep 15;392:51-55. doi: 10.1016/j.jns.2018.07.006. Epub 2018 Jul 10.
Leucine-rich alpha-2 glycoprotein (LRG) is a novel biomarker for inflammatory diseases. We evaluated the levels of LRG, interleukin (IL)-6, and tumor necrosis factor (TNF)-α in the cerebrospinal fluid (CSF) of children with meningitis.
CSF samples from 10 patients with bacterial meningitis (BM) and 10 with aseptic meningitis (AM) were evaluated. Samples from 10 patients with febrile status (FS) were used as controls. LRG levels were measured using a two-site enzyme immunoassay. IL-6 and TNF-α levels were measured using a multiplex bead-based assay. CSF examination of patients with BM at the convalescent stage was also conducted.
LRG and TNF-α levels in patients with BM, and IL-6 levels in patients with BM and AM showed significant increase compared with those in FS. Patients with BM at the convalescent stage showed significantly diminished LRG and IL-6 levels. LRG and IL-6 levels in CSF were indicated to be effective predictors for BM (LRG, AUC = 0.91; IL-6, AUC = 0.85). Only LRG levels showed a significant difference between patients with BM and AM (AUC = 0.78, P = 0.034).
LRG level could be a sensitive inflammatory biomarker for inflammatory diseases of the central nervous system, comparable with IL-6 level.
亮氨酸丰富α-2 糖蛋白（LRG）是一种新型的炎症性疾病生物标志物。我们评估了脑膜炎患儿脑脊液（CSF）中 LRG、白细胞介素（IL）-6 和肿瘤坏死因子（TNF）-α的水平。
评估了 10 例细菌性脑膜炎（BM）和 10 例无菌性脑膜炎（AM）患儿的 CSF 样本。10 例发热状态（FS）患儿的样本作为对照。使用双位点酶免疫测定法测量 LRG 水平。使用基于多重珠的测定法测量 IL-6 和 TNF-α 水平。还对 BM 患者的恢复期 CSF 进行了检查。
BM 患者的 LRG 和 TNF-α 水平，以及 BM 和 AM 患者的 IL-6 水平与 FS 相比均显著升高。恢复期 BM 患者的 LRG 和 IL-6 水平显著降低。CSF 中的 LRG 和 IL-6 水平被证明是 BM 的有效预测指标（LRG，AUC=0.91；IL-6，AUC=0.85）。仅 LRG 水平在 BM 和 AM 患者之间存在显著差异（AUC=0.78，P=0.034）。
LRG 水平可能是中枢神经系统炎症性疾病的敏感炎症生物标志物，与 IL-6 水平相当。
