Department of Physics, Bose Institute, 93/1, Acharya Prafulla Chandra Road, Kolkata 700009, India; Department of Physics, Brahmananda Keshab Chandra College, 111/2 B. T. Road, Bonhooghly, Kolkata 700108, India.
Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700064, India.
Biosens Bioelectron. 2018 Nov 15;119:10-17. doi: 10.1016/j.bios.2018.07.061. Epub 2018 Jul 30.
Fabrication of Molybdenum disulfide (MoS) nanostructures and surface functionalization with noble metal nano particles is an emerging field of research as it has potential applications in electronic devices and chemical sensing. Here we report application of gold nanoparticles (AuNPs) decorated MoS nanoflowers (Au-MoS NFs) as an efficient bio-sensor. MoS NFs, synthesized using green synthesis process, are further functionalized with AuNPs to tune their physical properties and make them more appropriate for biological applications. The abundant 'hot-spots' created by AuNPs through localization of electromagnetic field endows the Au-MoS hybrid structure as an excellent substrate for biochemical sensing through surface enhanced Raman scattering (SERS). The sensing efficiency of the SERS substrate is examined using Rh6G as probe molecule with concentration as low as 10 M. Main emphasis is given in detecting free bilirubin, an important component of human blood, using SERS technique. Au-MoS NF SERS substrate exhibits high sensitivity, stability and excellent reproducibility in sensing bilirubin from high level (10 M) to picomolar level. The concentration (C) dependent SERS intensity (I) is found to follow the general relationship I = C, with α ranging from 0.09 to 0.12. The substrate shows excellent selectivity and reliability while sensing of free bilirubin performed in human serum in the presence of crucial interferences such as dextrose, cholesterol and phosphate. In the present study, this Au-MoS hybrid offers a new potential biosensing technology for free bilirubin detection and is anticipated to be applied for clinic diagnosis.
二硫化钼（MoS）纳米结构的制造以及与贵金属纳米粒子的表面功能化是一个新兴的研究领域，因为它在电子设备和化学传感中有潜在的应用。在这里，我们报告了金纳米粒子（AuNPs）修饰的 MoS 纳米花（Au-MoS NFs）作为高效生物传感器的应用。使用绿色合成工艺合成的 MoS NFs 进一步用 AuNPs 功能化，以调整其物理性质，使其更适合生物应用。AuNPs 通过电磁场的局域化产生的丰富“热点”使 Au-MoS 杂化结构成为通过表面增强拉曼散射（SERS）进行生化传感的优异基底。使用 Rh6G 作为探针分子，以低至 10 M 的浓度检测 SERS 基底的检测效率。主要重点是使用 SERS 技术检测胆红素，胆红素是人体血液的重要组成部分。Au-MoS NF SERS 基底在检测胆红素时表现出高灵敏度、稳定性和出色的重现性，可检测高浓度（10 M）至皮摩尔级的胆红素。发现 C 依赖性 SERS 强度（I）遵循一般关系 I = C，其中 α 范围为 0.09 至 0.12。在存在葡萄糖、胆固醇和磷酸盐等关键干扰物的情况下，在人血清中进行游离胆红素检测时，该基底表现出出色的选择性和可靠性。在本研究中，这种 Au-MoS 杂化材料为游离胆红素的检测提供了一种新的潜在生物传感技术，并有望应用于临床诊断。
