Ivanovo State University of Chemistry and Technology, 153000 Ivanovo, Sheremetevskii ave. 7, Russia.
Ivanovo State University of Chemistry and Technology, 153000 Ivanovo, Sheremetevskii ave. 7, Russia.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Jan 5;206:160-164. doi: 10.1016/j.saa.2018.08.009. Epub 2018 Aug 7.
Present contribution describes the UV-Vis study of the mixture of Cu(II) ions, pyridoxal 5'-phosphate nicotinoyl hydrazone and DNA. Neither free hydrazone nor its copper(II) complex interacts with DNA under the given concentration conditions. The changes in the UV-Vis spectra of the mixture containing metal complex and DNA are caused by partial dissociation of the coordination compound and complexation of the released Cu(II) ions with DNA. This result was obtained by the analysis of a number of the reactions that could occur in the solution of Cu(II) ions, buffer components (namely, Tris), ligand (hydrazone), and DNA.
本研究描述了 Cu(II)离子、吡哆醛 5'-磷酸烟酰腙和 DNA 混合物的紫外可见光谱研究。在给定浓度条件下，游离腙及其铜(II)配合物均不与 DNA 相互作用。混合物中金属配合物和 DNA 的紫外可见光谱的变化是由于部分配合物的解离和释放的 Cu(II)离子与 DNA 的配位反应引起的。通过分析溶液中可能发生的一系列反应，包括 Cu(II)离子、缓冲成分（即 Tris）、配体（腙）和 DNA，得到了这一结果。
